The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.
The switching mechanism of the bacterial rotary motor combines tight regulation with inherent flexibility.
复制标题
DOI：
10.15252/embj.2020104683
复制
发表时间：
2021-03-15
期刊：
影响因子：
--
通讯作者：
Eisenbach M
中科院分区：
文献类型：
--
作者：
Afanzar O;Di Paolo D;Eisenstein M;Levi K;Plochowietz A;Kapanidis AN;Berry RM;Eisenbach M
调节开关广泛存在于许多生物系统中。其中独特的是，细菌鞭毛马达的开关不是开/关开关，而是控制马达的旋转方向，以响应信号蛋白CheY的结合。尽管有广泛的研究，但这种开关背后的分子机制仍然很不清楚。在这里，我们解析了E中开关处的三个CheY结合位点中的每一个的功能。大肠杆菌，以及它们对磷酸化和乙酰化的不同依赖性。基于此，我们提出，CheY电机开关活动是加强后，绑定到第一个网站。增强的CheY与第二个位点的结合产生不稳定的转换，同时使CheY与第三个位点结合，这是一个稳定转换状态的事件。因此，该机制实现了电机严格调节与固有开关灵活性的独特组合。CheY与E.大肠杆菌鞭毛马达将细菌的游动行为从游动转变为翻滚，从而实现趋化性。
Regulatory switches are wide spread in many biological systems. Uniquely among them, the switch of the bacterial flagellar motor is not an on/off switch but rather controls the motor’s direction of rotation in response to binding of the signaling protein CheY. Despite its extensive study, the molecular mechanism underlying this switch has remained largely unclear. Here, we resolved the functions of each of the three CheY‐binding sites at the switch in E. coli, as well as their different dependencies on phosphorylation and acetylation of CheY. Based on this, we propose that CheY motor switching activity is potentiated upon binding to the first site. Binding of potentiated CheY to the second site produces unstable switching and at the same time enables CheY binding to the third site, an event that stabilizes the switched state. Thereby, this mechanism exemplifies a unique combination of tight motor regulation with inherent switching flexibility. A three‐step mechanism of CheY binding to the E. coli flagellar motor switches bacterial swimming behavior from swimming to tumbling to enable chemotaxis.
登录
查看更多内容
影响因子：
5.6
作者：
Bren, A;Eisenbach, M
通讯作者：
Eisenbach, M
影响因子：
3.6
作者：
Liarzi, Orna;Barak, Rina;Eisenbach, Michael
通讯作者：
Eisenbach, Michael
影响因子：
3.2
作者：
Dyer, Collin M.;Dahlquist, Frederick W.
通讯作者：
Dahlquist, Frederick W.
影响因子：
3.2
作者：
Barak, R;Abouhamad, WN;Eisenbach, M
通讯作者：
Eisenbach, M
影响因子：
64.5
作者：
Laslo, Peter;Spooner, Chauncey J.;Singh, Harinder
通讯作者：
Singh, Harinder