MORC1 represses transposable elements in the mouse male germline.
MORC1 represses transposable elements in the mouse male germline.
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DOI：
10.1038/ncomms6795
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发表时间：
2014-12-12
影响因子：
16.6
通讯作者：
Jacobsen, Steven E.
中科院分区：
文献类型：
--
作者：
Pastor, William A.;Stroud, Hume;Nee, Kevin;Liu, Wanlu;Pezic, Dubravka;Manakov, Sergei;Lee, Serena A.;Moissiard, Guillaume;Zamudio, Natasha;Bourc'his, Deborah;Aravin, Alexei A.;Clark, Amander T.;Jacobsen, Steven E.
微小支原体(MORC)家族的GHKL-ATPase广泛存在于原核和真核生物中，但其功能尚不清楚。在拟南芥的遗传筛选中，已经发现MORC基因是转座子和其他DNA甲基化和沉默基因的重要抑制基因。MORC1基因缺陷的小鼠之前被发现表现出男性特有的生殖细胞丢失和不育。在这里，我们表明MORC1在男性生殖系中负责转座子抑制，其模式类似于在缺乏DNA甲基转移酶同源物DNMT3L的生殖细胞中观察到的模式。Morc1突变体在特定类型的转座子的DNA甲基化建立过程中表现出高度局部性的缺陷，这与这些位置的转座子沉默失败有关。我们的结果确定MORC1是在全球从头甲基化期间男性生殖细胞表观遗传格局的一个重要的新调节因子。微小支原体(MORC)家族的GHKL-ATPase是拟南芥转座子和其他DNA甲基化沉默基因的重要抑制因子。在这里，作者表明，MORC1负责抑制和甲基化特定类别的转座子在小鼠雄性生殖系。
The Microrchidia (Morc) family of GHKL ATPases are present in a wide variety of prokaryotic and eukaryotic organisms but are of largely unknown function. Genetic screens in Arabidopsis thaliana have identified Morc genes as important repressors of transposons and other DNA-methylated and silent genes. MORC1-deficient mice were previously found to display male-specific germ cell loss and infertility. Here we show that MORC1 is responsible for transposon repression in the male germline in a pattern that is similar to that observed for germ cells deficient for the DNA methyltransferase homologue DNMT3L. Morc1 mutants show highly localized defects in the establishment of DNA methylation at specific classes of transposons, and this is associated with failed transposon silencing at these sites. Our results identify MORC1 as an important new regulator of the epigenetic landscape of male germ cells during the period of global de novo methylation. The Microrchidia (Morc) family of GHKL ATPases are important repressors of transposons and other DNA-methylated and silent genes in A. thaliana. Here, the authors show that MORC1 is responsible for repression and methylation of specific classes of transposons in the mouse male germline.
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影响因子：
12.3
作者：
Langmead B;Schatz MC;Lin J;Pop M;Salzberg SL
通讯作者：
Salzberg SL
影响因子：
3.5
作者：
Inoue, N;Hess, KD;Zinn, AR
通讯作者：
Zinn, AR
影响因子：
14.9
作者：
Karolchik D;Barber GP;Casper J;Clawson H;Cline MS;Diekhans M;Dreszer TR;Fujita PA;Guruvadoo L;Haeussler M;Harte RA;Heitner S;Hinrichs AS;Learned K;Lee BT;Li CH;Raney BJ;Rhead B;Rosenbloom KR;Sloan CA;Speir ML;Zweig AS;Haussler D;Kuhn RM;Kent WJ
通讯作者：
Kent WJ
影响因子：
10.5
作者：
Chotalia, Mita;Smallwood, Sebastien A.;Kelsey, Gavin
通讯作者：
Kelsey, Gavin
影响因子：
10.5
作者：
Kuramochi-Miyagawa, Satomi;Watanabe, Toshiaki;Nakano, Toru
通讯作者：
Nakano, Toru