Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance.
Validation of a rapid, saliva-based, and ultra-sensitive SARS-CoV-2 screening system for pandemic-scale infection surveillance.
复制标题
DOI：
10.1038/s41598-022-08263-4
复制
发表时间：
2022-04-08
影响因子：
4.6
通讯作者：
Kõks S
中科院分区：
文献类型：
--
作者：
Dewhurst RE;Heinrich T;Watt P;Ostergaard P;Marimon JM;Moreira M;Houldsworth PE;Rudrum JD;Wood D;Kõks S
由于没有实现全面全球疫苗覆盖和持久免疫的现实前景，COVID-19等大流行的控制将需要大规模、快速识别和隔离感染个体，以限制进一步传播。在这里，我们描述了一个自动化的、高通量的综合筛选平台，结合基于唾液的环介导等温扩增（LAMP）技术，设计用于群体规模的SARS-CoV-2 RNA感染载体的敏感检测。该监测系统的核心是“哨兵”检测仪器，它能够在唾液样本采集后25分钟内报告结果，每小时的吞吐量高达3840个结果。它结合了随机间隔的样品连续流加载，以经济有效地调整测试需求的波动。我们的基于唾液的RT-LAMP技术在自动化LAMP仪器“哨兵”上进行了独立验证，发现与RT-PCR比较试验相比，灵敏度为98.7%，特异性为97.6%，准确性为98%，证实了其用于监测筛查的适用性。这一哨点监测系统提供了一种可行和可扩展的方法来补充疫苗接种，遏制COVID-19变体的传播，并控制未来的大流行以拯救生命。
Without any realistic prospect of comprehensive global vaccine coverage and lasting immunity, control of pandemics such as COVID-19 will require implementation of large-scale, rapid identification and isolation of infectious individuals to limit further transmission. Here, we describe an automated, high-throughput integrated screening platform, incorporating saliva-based loop-mediated isothermal amplification (LAMP) technology, that is designed for population-scale sensitive detection of infectious carriers of SARS-CoV-2 RNA. Central to this surveillance system is the “Sentinel” testing instrument, which is capable of reporting results within 25 min of saliva sample collection with a throughput of up to 3840 results per hour. It incorporates continuous flow loading of samples at random intervals to cost-effectively adjust for fluctuations in testing demand. Independent validation of our saliva-based RT-LAMP technology on an automated LAMP instrument coined the “Sentinel”, found 98.7% sensitivity, 97.6% specificity, and 98% accuracy against a RT-PCR comparator assay, confirming its suitability for surveillance screening. This Sentinel surveillance system offers a feasible and scalable approach to complement vaccination, to curb the spread of COVID-19 variants, and control future pandemics to save lives.
登录
查看更多内容
DOI：
10.1016/j.ijid.2021.04.018
发表时间：
2021-06
期刊：
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
影响因子：
--
作者：
Inaba M;Higashimoto Y;Toyama Y;Horiguchi T;Hibino M;Iwata M;Imaizumi K;Doi Y
通讯作者：
Doi Y
影响因子：
3.7
作者：
Lista MJ;Matos PM;Maguire TJA;Poulton K;Ortiz-Zapater E;Page R;Sertkaya H;Ortega-Prieto AM;Scourfield E;O'Byrne AM;Bouton C;Dickenson RE;Ficarelli M;Jimenez-Guardeño JM;Howard M;Betancor G;Galao RP;Pickering S;Signell AW;Wilson H;Cliff P;Kia Ik MT;Patel A;MacMahon E;Cunningham E;Doores K;Agromayor M;Martin-Serrano J;Perucha E;Mischo HE;Shankar-Hari M;Batra R;Edgeworth J;Zuckerman M;Malim MH;Neil S;Martinez-Nunez RT
通讯作者：
Martinez-Nunez RT
影响因子：
5.7
作者：
Huang, Wei E.;Lim, Boon;Cui, Zhanfeng
通讯作者：
Cui, Zhanfeng
影响因子：
11.7
作者：
Lau, H.;Khosrawipour, T.;Khosrawipour, V
通讯作者：
Khosrawipour, V
影响因子：
11.8
作者：
Bullard, Jared;Dust, Kerry;Poliquin, Guillaume
通讯作者：
Poliquin, Guillaume