CrfP, a fratricide protein, contributes to natural transformation in Streptococcus suis.
CrfP, a fratricide protein, contributes to natural transformation in Streptococcus suis.
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CrfP是一种杀兄弟蛋白，有助于猪链球菌的自然转化。
DOI：
10.1186/s13567-021-00917-x
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发表时间：
2021-03-24
影响因子：
4.4
通讯作者：
Yao H
中科院分区：
文献类型：
--
作者：
Zhu Y;Ma J;Zhang Y;Zhong X;Bai Q;Dong W;Pan Z;Liu G;Zhang C;Yao H
猪链球菌（S. suis）是一种重要的人畜共患病原体，可引起宿主败血症、脑膜炎和链球菌中毒性休克样综合征，最近的研究表明，猪链球菌可能具有自然遗传转化能力。转化是DNA水平转移的重要机制，但影响转化过程的一些因素还有待进一步探讨。在进入能力状态后，链球菌会刺激能力相关基因的转录，这些基因负责外源DNA的结合、摄取和加工。在这项研究中，我们进行了保守启动子基序和qRT-PCR分析，发现CrfP是一种新的鼠蛋白水解酶，在猪链球菌中广泛存在，并通过ComX控制的过程受到肽信息素的激活。生物信息学分析显示，CrfP由一个CHAP水解酶结构域和两个细菌Src同源3-结合（SH3b）结构域组成。进一步的鉴定表明，CrfP可以输出到不同血清型的胞外细菌细胞和裂解型猪链球菌中，并通过透射电镜和浊度试验验证了这一发现。为了研究CrfP在体内的潜在作用，构建了一个基因缺失突变体（ΔcrfP）。CrfP的失活并没有阻止自然转化过程，反而明显降低了有效转化率。总的来说，这些发现提供了证据表明CrfP对猪链球菌血清2型能力很重要。在线版本包含补充材料，可在10.1186/s13567-021-00917-x获得。
Streptococcus suis (S. suis) is an important zoonotic pathogen that causes septicaemia, meningitis and streptococcal toxic shock-like syndrome in its host, and recent studies have shown that S. suis could be competent for natural genetic transformation. Transformation is an important mechanism for the horizontal transfer of DNA, but some elements that affect the transformation process need to be further explored. Upon entering the competent state, Streptococcus species stimulate the transcription of competence-related genes that are responsible for exogenous DNA binding, uptake and processing. In this study, we performed conserved promoter motif and qRT-PCR analyses and identified CrfP as a novel murein hydrolase that is widespread in S. suis and stimulated with a peptide pheromone in the competent state through a process controlled by ComX. A bioinformatics analysis revealed that CrfP consists of a CHAP hydrolase domain and two bacterial Src homology 3-binding (SH3b) domains. Further characterization showed that CrfP could be exported to extracellular bacterial cells and lytic S. suis strains of different serotypes, and this finding was verified by TEM and a turbidity assay. To investigate the potential effect of CrfP in vivo, a gene-deletion mutant (ΔcrfP) was constructed. Instead of stopping the natural transformation process, the inactivation of CrfP clearly reduced the effective transformation rate. Overall, these findings provide evidence showing that CrfP is important for S. suis serovar 2 competence. The online version contains supplementary material available at 10.1186/s13567-021-00917-x.
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影响因子：
4.5
作者：
Chewapreecha C;Marttinen P;Croucher NJ;Salter SJ;Harris SR;Mather AE;Hanage WP;Goldblatt D;Nosten FH;Turner C;Turner P;Bentley SD;Parkhill J
通讯作者：
Parkhill J
影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
DOI：
10.1007/s10096-016-2616-x
发表时间：
2016-06
期刊：
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
影响因子：
--
作者：
Bojarska A;Molska E;Janas K;Skoczyńska A;Stefaniuk E;Hryniewicz W;Sadowy E
通讯作者：
Sadowy E
影响因子：
3.6
作者：
Håvarstein, LS;Martin, B;Claverys, JP
通讯作者：
Claverys, JP
影响因子：
3.2
作者：
Berg, Kari Helene;Ohnstad, Hilde Solheim;Havarstein, Leiv Sigve
通讯作者：
Havarstein, Leiv Sigve