An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation.
An ATP-dependent mechanism mediates intercellular calcium signaling in bone cell network under single cell nanoindentation.
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DOI：
10.1016/j.ceca.2009.12.005
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发表时间：
2010-03
期刊：
影响因子：
4
通讯作者：
Guo XE
中科院分区：
文献类型：
--
作者：
Huo B;Lu XL;Costa KD;Xu Q;Guo XE
为了研究细胞间隙连接和细胞外ATP扩散在骨组织中骨细胞钙信号传递中的作用，采用微接触打印和自组装单层技术构建了体外骨细胞网络。在该网络中，相邻的细胞通过功能缝隙连接相互连接。位于网络中心的单个细胞通过使用AFM纳米压头进行机械刺激。记录并分析骨细胞网络的细胞内钙([Ca+]i)反应。在未处理组中，在40%的测试中观察到从刺激细胞到邻近细胞的钙扩散。当细胞间缝隙连接被阻断时，这一百分比没有明显差异。然而，在细胞外ATP途径阻滞组，这一数字下降到10%。当缝隙连接和ATP通路都被阻断时，细胞间钙波被消除。当内质网中的细胞内钙储备耗尽时，凹陷细胞可以产生钙瞬变，但不能向邻近细胞传播[Ca~(2+)]i信号。去除细胞外钙源后，细胞网络中未检测到[Ca~(2+)]i反应。这些发现确定了钙信号在骨细胞网络中传播的生化途径。
To investigate the roles of intercellular gap junctions and extracellular ATP diffusion in bone cell calcium signaling propagation in bone tissue, in vitro bone cell networks were constructed by using microcontact printing and self-assembled monolayer technologies. In the network, neighboring cells were interconnected through functional gap junctions. A single cell at the center of the network was mechanically stimulated by using an AFM nanoindenter. Intracellular calcium ([Ca2+]i) responses of the bone cell network were recorded and analyzed. In the untreated groups, calcium propagation from the stimulated cell to neighboring cells was observed in 40% of the tests. No significant difference was observed in this percentage when the intercellular gap junctions were blocked. This number, however, decreased to 10% in the extracellular ATP-pathway-blocked group. When both the gap junction and ATP pathways were blocked, intercellular calcium waves were abolished. When the intracellular calcium store in ER was depleted, the indented cell can generate calcium transients, but no [Ca2+]i signal can be propagated to the neighboring cells. No [Ca2+]i response was detected in the cell network when the extracellular calcium source was removed. These findings identified the biochemical pathways involved in the calcium signaling propagation in bone cell networks.
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DOI：
10.1152/ajplung.1999.276.6.l1018
发表时间：
1999-06-01
影响因子：
4.9
作者：
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影响因子：
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作者：
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