Similar upstream regulatory elements of genes that encode the two largest subunits of RNA polymerase II in Saccharomyces cerevisiae.
Similar upstream regulatory elements of genes that encode the two largest subunits of RNA polymerase II in Saccharomyces cerevisiae.
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酿酒酵母中编码 RNA 聚合酶 II 两个最大亚基的基因的类似上游调控元件。
DOI：
10.1093/nar/24.22.4543
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发表时间：
1996
影响因子：
14.9
通讯作者：
James D. Friesen
中科院分区：
文献类型：
--
作者：
D. B. Jansma;Jacques Archambault;Omid Mostachfi;James D. Friesen
我们已经确定了位置的顺式作用元件是重要的RPO 21和RPO 22，基因编码的两个最大的亚基的RNA聚合酶II（RNAPII）在酿酒酵母的表达。测试了RPO 21和RPO 22上游区域中的一系列5 '端缺失和核苷酸取代对这些基因的lacZ融合体表达的影响。RPO 21中-723至-693的序列缺失，破坏了两个Reb 1 p结合位点和一个Abf 1 p结合位点，导致表达降低10倍。这些位点下游的富含T的区域对于表达也很重要。RPO 22上游-437至-392的序列缺失导致表达减少30倍，表明该区域的Reb 1 p和Abf 1 p结合位点对RPO 22表达很重要，就像这些位点下游富含T的序列一样。RPO 21和RPO 22上游区域能够在体外与Reb 1 p和Abf 1 p相互作用（凝胶迁移率变动测定）。RPO 21和RPO 22上游区域元件的类型和组织的相似性表明，这些基因的表达可能是协同调节的。
We have determined the location of cis-acting elements that are important for the expression of RPO21 and RPO22, genes that encode the two largest subunits of RNA polymerase II (RNAPII) in Saccharomyces cerevisiae. A series of 5'-end deletions and nucleotide substitutions in the upstream regions of RPO21 and RPO22 were tested for their effect on the expression of lacZ fusions of these genes. Deletion of sequences from -723 to -693 in RPO21, which disrupted two Reb1p-binding sites and an Abf1p-binding site, resulted in a 10-fold decrease in expression. A T-rich region downstream of these sites was also important for expression. Deletion of sequences from -437 to -392 in the RPO22-upstream, which resulted in a 30-fold decrease in expression, indicated that the Reb1p- and Abf1p-binding sites in this region were important for RPO22 expression, as was a T-rich sequence immediately downstream of these sites. The RPO21 and RPO22 upstream regions were capable of interacting in vitro (gel-mobility-shift assays) with Reb1p and Abf1p. The similarities in the type and organization of elements in the upstream regions of RPO21 and RPO22 suggest that expression of these genes may be regulated coordinately.
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原核和真核RNA聚合酶具有同源的核心亚基。
DOI：
10.1073/pnas.84.5.1192
发表时间：
1987
影响因子：
11.1
作者：
Sweetser,D;Nonet,M;Young,RA
通讯作者：
Young,RA
酵母烯醇化酶基因 ENO1 的上游抑制序列是一个复杂的调控元件，可结合包括 REB1 在内的多种反式作用因子。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Carmen,AA;Holland,MJ
通讯作者：
Holland,MJ
影响因子：
11.4
作者：
IYER, V;STRUHL, K
通讯作者：
STRUHL, K
影响因子：
10.5
作者：
CHASMAN, DI;LUE, NF;KORNBERG, RD
通讯作者：
KORNBERG, RD