Control of Co1E2 plasmid replication: negative regulation of the expression of the plasmid-specified initiator protein, Rep, at a posttranscriptional step
Control of Co1E2 plasmid replication: negative regulation of the expression of the plasmid-specified initiator protein, Rep, at a posttranscriptional step
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Co1E2 质粒复制的控制：转录后步骤中质粒特异起始蛋白 Rep 表达的负调控
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
T. Itoh
中科院分区：
文献类型：
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作者：
H. Yasueda;S. Takechi;T. Sugiyama;T. Itoh
CoIE 2的印加基因参与拷贝数控制和不亲和性。在印加基因周围鉴定了两个启动子。必需质粒编码的起始蛋白（Rep）的mRNA的转录主要起始于Rep蛋白起始密码子上游约140的位点。第二个转录本（RNA 1）约115个核苷酸，具有两个茎环结构，与Rep mRNA的5′端非翻译区完全互补。通过使用CoIE 2的rep基因和大肠杆菌的lacZ基因的翻译和转录融合，显示印加基因产物在转录后步骤调节rep基因的表达。结果还表明，印加基因产物的靶点是Rep mRNA的5′非翻译区。本文报道的缺失分析表明，Rep蛋白起始密码子上游约17至70的区域和编码框内的另一个区域对于Rep蛋白的有效生产是重要的。这表明，一些额外的序列元件以外的起始密码子和Shine-Dalgarno区和/或二级结构的Rep mRNA的Rep蛋白的有效生产所需的。这些结果表明，RNA I是Rep mRNA的反义RNA，并暗示它可能在翻译的起始步骤通过隔离这些额外的序列元件和/或通过破坏RNA二级结构来调节rep基因的表达。我们建议RNA I代表印加基因产物。
The incA gene of CoIE2 is involved in the copy number control and incompatibility. Two promoters were identified around the incA gene. Transcription of the mRNA for the essential plasmid-coded initiator protein (Rep) mainly starts at a site about 140 by upstream of the initiation codon of the Rep protein. The second transcript (RNA 1) of about 115 nucleotides with two stem-and-loop structures is entirely complementary to the 5′ untranslated region of the Rep mRNA. By using translational and transcriptional fusions of the rep gene of CoIE2 and the lacZ gene of Escherichia coli, the incA gene product was shown to regulate expression of the rep gene at a posttranscriptional step. The results also suggest that the target of the incA gene product is the 5′ untranslated region of the Rep mRNA. Deletion analyses reported here show that a region(s) about 17 to 70 by upstream of the initiation codon of the Rep protein and another region inside the coding frame are important for efficient production of the Rep protein. This suggests that some additional sequence elements other than the initiation codon and the Shine-Dalgarno region and/or a secondary structure of the Rep mRNA are required for efficient production of the Rep protein. These results show that RNA I is an antisense RNA for the Rep mRNA and imply that it might regulate expression of the rep gene at the initiation step of translation by sequestering such additional sequence elements and/or by disrupting RNA secondary structure. We propose that RNA I represents the incA gene product.
通过基因 V、VII、IX 和 VIII 起始位点的差异活性对噬菌体 f1 基因表达进行翻译控制。
影响因子：
5.6
作者：
Blumer,KJ;Ivey,MR;Steege,DA
通讯作者：
Steege,DA
影响因子：
10.5
作者：
KRINKE, L;WULFF, DL
通讯作者：
WULFF, DL
新型多功能质粒载体，用于表达由与编码 β-半乳糖苷酶酶活性羧基末端部分的 lacZ 基因序列融合的克隆基因编码的杂合蛋白。
影响因子：
3.5
作者：
Shapira,SK;Chou,J;Richaud,FV;Casadaban,MJ
通讯作者：
Casadaban,MJ