Cytokine signatures in chronic fatigue syndrome patients: a Case Control Study and the effect of anakinra treatment.
Cytokine signatures in chronic fatigue syndrome patients: a Case Control Study and the effect of anakinra treatment.
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DOI：
10.1186/s12967-017-1371-9
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发表时间：
2017-12-29
影响因子：
7.4
通讯作者：
van der Meer JWM
中科院分区：
文献类型：
--
作者：
Roerink ME;Knoop H;Bronkhorst EM;Mouthaan HA;Hawinkels LJAC;Joosten LAB;van der Meer JWM
几十年来，细胞因子紊乱被认为与慢性疲劳综合征/肌痛性脑脊髓炎（CFS/ME）有关。50名女性CFS患者被纳入一项研究，研究白细胞介素-1受体拮抗剂阿那白滞素或安慰剂在4周内的作用。在治疗前和治疗后立即从患者中采集EDTA血浆。在基线时，同时从48名年龄匹配的健康女性邻居对照中收集血浆样本。使用基于“邻近延伸试验”（PEA）的免疫测定法，在1 μL样本中平行测定一组92种炎症标志物。由于转化生长因子β（TGF-β）和白细胞介素-1受体拮抗剂（IL-1 Ra）不包括在该平台中，因此使用ELISA测量这些细胞因子。在CFS/ME患者中，IL-12 p40和CSF-1的“标准化蛋白表达”值显著更高（p值分别为0.0042和0.049）。此外，使用LASSO回归，47个标志物的组合产生了校正AUC为0.73的预测模型。经过多次测试校正后，阿那白滞素对循环细胞因子没有影响。TGF-β在患者和对照组之间没有差异。总之，本研究证明，除了一组预测性生物标志物外，CFS/ME患者中IL-12 p40和CSF-1浓度增加。阿那白滞素对IL-1 Ra以外的循环细胞因子无影响。 试用注册：ClinicalTrials.gov标识符：NCT 02108210，注册日期2014年4月本文的在线版本（10.1186/s12967-017-1371-9）包含补充材料，可供授权用户使用。
Cytokine disturbances have been suggested to be associated with the Chronic Fatigue Syndrome/Myalgic encephalomyelitis (CFS/ME) for decades. Fifty female CFS patients were included in a study on the effect of the interleukin-1-receptor antagonist anakinra or placebo during 4 weeks. EDTA plasma was collected from patients before and directly after treatment. At baseline, plasma samples were collected at the same time from 48 healthy, age-matched female neighborhood controls. A panel of 92 inflammatory markers was determined in parallel in 1 μL samples using a ‘proximity extension assay’ (PEA) based immunoassay. Since Transforming growth factor beta (TGF-β) and interleukin-1 receptor antagonist (IL-1Ra) were not included in this platform, these cytokines were measured with ELISA. In CFS/ME patients, the ‘normalized protein expression’ value of IL-12p40 and CSF-1 was significantly higher (p value 0.0042 and 0.049, respectively). Furthermore, using LASSO regression, a combination of 47 markers yielded a prediction model with a corrected AUC of 0.73. After correction for multiple testing, anakinra had no effect on circulating cytokines. TGF-β did not differ between patients and controls. In conclusion, this study demonstrated increased IL-12p40 and CSF-1 concentrations in CFS/ME patients in addition to a set of predictive biomarkers. There was no effect of anakinra on circulating cytokines other than IL-1Ra. Trial Registration: ClinicalTrials.gov Identifier: NCT02108210, Registered April 2014 The online version of this article (10.1186/s12967-017-1371-9) contains supplementary material, which is available to authorized users.
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