Mapping determinants of gene expression plasticity by genetical genomics in C. elegans.
Mapping determinants of gene expression plasticity by genetical genomics in C. elegans.
复制标题
在秀丽隐杆线虫中，基因基因组学对基因表达可塑性的映射决定因素。
DOI：
10.1371/journal.pgen.0020222
复制
发表时间：
2006-12-29
期刊：
影响因子：
4.5
通讯作者：
Kammenga, Jan E.
中科院分区：
文献类型：
--
作者：
Li, Yang;Alvarez, Olga Alda;Gutteling, Evert W.;Tijsterman, Marcel;Fu, Jingyuan;Riksen, Joost A. G.;Hazendonk, Esther;Prins, Pjotr;Plasterk, Ronald H. A.;Jansen, Ritsert C.;Breitling, Rainer;Kammenga, Jan E.
最近的遗传基因组学研究提供了基因调控网络的亲密的看法。遗传上不同的个体之间的基因表达变异已被定位到因果调节区，称为表达数量性状位点。环境诱导的基因表达的可塑性反应是否也表现出遗传差异尚未研究。在这里，我们表明，由16 °C和24 °C的温度诱导的差异表达在秀丽隐杆线虫重组近交系中具有很强的遗传成分，该重组近交系来源于菌株CB 4856（夏威夷）和N2（布里斯托）之间的杂交。不少于59%的308个反式作用基因表现出显着的eQTL环境互作，这里称为可塑性数量性状位点。相比之下，只有8%的估计188顺式作用基因表现出这种相互作用。这表明基因表达的可塑性反应的遗传差异在很大程度上是受反式调节的。这种调节分布在许多不同的调节因子中。然而，对于一组转基因，我们发现了一个共同的主调节因子的突出证据：在24 °C下出现了66个共调节基因的转带。我们的研究结果表明，广泛的遗传变异的差异表达对环境的影响，并展示了潜在的遗传基因组学映射的分子决定因素的表型可塑性。环境变化会诱导基因的差异表达，这是广泛记载的，但它是未知的，这些模式的环境诱导的表达可塑性是如何遗传的，以及它们如何不同的生物物种的遗传不同的个体。本文作者使用了线虫C的重组近交系。来源于最初在布里斯托（英国）和夏威夷收集的亲本品系的线虫，并在两种不同温度下测量全基因组基因表达。利用为数量性状基因座作图开发的统计分析工具，他们发现了基因在对温度变化的塑性反应中具有遗传决定的差异。发现它们中的大多数受不同基因组位置的基因调控（反式调控）。一个引人注目的观察结果是一组66个基因，它们共享一个共同的潜在调节因子，可能与生育可塑性的差异有关。这些结果表明，不同基因型对环境变化的不同反应是普遍存在的。由于所有物种都受到环境变化的影响，无论是在个体还是进化的时间尺度上，作者的工作要求研究其他生物体中基因调控可塑性的遗传成分，以增强对驱动进化适应的环境力量的理解。
Recent genetical genomics studies have provided intimate views on gene regulatory networks. Gene expression variations between genetically different individuals have been mapped to the causal regulatory regions, termed expression quantitative trait loci. Whether the environment-induced plastic response of gene expression also shows heritable difference has not yet been studied. Here we show that differential expression induced by temperatures of 16 °C and 24 °C has a strong genetic component in Caenorhabditis elegans recombinant inbred strains derived from a cross between strains CB4856 (Hawaii) and N2 (Bristol). No less than 59% of 308 trans-acting genes showed a significant eQTL-by-environment interaction, here termed plasticity quantitative trait loci. In contrast, only 8% of an estimated 188 cis-acting genes showed such interaction. This indicates that heritable differences in plastic responses of gene expression are largely regulated in trans. This regulation is spread over many different regulators. However, for one group of trans-genes we found prominent evidence for a common master regulator: a transband of 66 coregulated genes appeared at 24 °C. Our results suggest widespread genetic variation of differential expression responses to environmental impacts and demonstrate the potential of genetical genomics for mapping the molecular determinants of phenotypic plasticity. It is widely documented that environmental changes will induce differential expression of genes, yet it is unknown how these patterns of environment-induced expression plasticity are inherited and how they differ between genetically divergent individuals of a biological species. In this paper the authors used recombinant inbred lines of the nematode worm C. elegans that were derived from parental lines originally collected in Bristol (United Kingdom) and Hawaii, and measured genome-wide gene expression at two different temperatures. Using statistical analysis tools developed for quantitative trait locus mapping, they found genes with genetically determined differences in their plastic response to temperature changes. A majority of them were found to be regulated by genes at a different genome position (regulated in trans). A striking observation was a group of 66 genes that share a common potential regulator and may be related to differences in fertility plasticity. These results show that differential responses of different genotypes to environmental changes are widespread. Because all species are subjected to environmental change, both at individual and evolutionary time scales, the authors' work calls for studying the heritable component of plasticity of gene regulation in other organisms to enhance understanding of the environmental forces that drive evolutionary adaptation.
登录
查看更多内容
影响因子：
3.3
作者：
Fu, JY;Jansen, RC
通讯作者：
Jansen, RC
影响因子：
56.9
作者：
Nelson, LS;Rosoff, ML;Li, C
通讯作者：
Li, C
DOI：
10.1002/dvg.1020150310
发表时间：
1994-01-01
期刊：
DEVELOPMENTAL GENETICS
影响因子：
--
作者：
CREWS, D;BERGERON, JM;WIBBELS, T
通讯作者：
WIBBELS, T
影响因子：
--
作者：
Kraus, PR;Boily, MJ;Heitman, J
通讯作者：
Heitman, J
影响因子：
5.8
作者：
Maere, S;Heymans, K;Kuiper, M
通讯作者：
Kuiper, M