Resource partitioning and amino acid assimilation in a terrestrial geothermal spring.
Resource partitioning and amino acid assimilation in a terrestrial geothermal spring.
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DOI：
10.1038/s41396-023-01517-7
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发表时间：
2023-11
期刊：
影响因子：
11
通讯作者：
Hungate, Bruce A.
中科院分区：
文献类型：
--
作者：
Lai, Dengxun;Hedlund, Brian P.;Mau, Rebecca L.;Jiao, Jian-Yu;Li, Junhui;Hayer, Michaela;Dijkstra, Paul;Schwartz, Egbert;Li, Wen-Jun;Dong, Hailiang;Palmer, Marike;Dodsworth, Jeremy A.;Zhou, En-Min;Hungate, Bruce A.
高温地热泉宿主简化的微生物群落;然而，单个微生物的活动及其在自然界碳循环中的作用尚不清楚。本文采用定量稳定同位素探针（qSIP）技术对腾冲公小舍温泉74 °C沉积物中13 C-乙酸盐和13 C-天冬氨酸盐同化为DNA的过程进行了研究。这揭示了一个社区范围内的偏好天冬氨酸和天冬氨酸掺入DNA和天冬氨酸利用者在标记过程中的增殖之间的紧密耦合。13 C掺入到DNA和孵化过程中的类群丰度的变化表明强大的资源分区和天冬氨酸掺入显着的系统发育信号。在qSIP鉴定的活性扩增子序列变异体（ASVs）中，大部分与公小舍温泉或附近温泉的基因组匹配，平均核苷酸相似性为99.4%。对应于天冬氨酸主要利用者的基因组较小，几乎普遍编码极性氨基酸ABC转运蛋白，并具有指示更快生长速率的密码子偏好。最活跃的ASVs同化这两种基板并不丰富，这表明了一个重要的作用，罕见的生物圈在社会响应有机碳添加。广泛纳入天冬氨酸到DNA乙酸的温泉社区可能反映了动态循环的细胞裂解产物原位或基板在季风降雨期间交付，并可能反映N限制。
High-temperature geothermal springs host simplified microbial communities; however, the activities of individual microorganisms and their roles in the carbon cycle in nature are not well understood. Here, quantitative stable isotope probing (qSIP) was used to track the assimilation of 13C-acetate and 13C-aspartate into DNA in 74 °C sediments in Gongxiaoshe Hot Spring, Tengchong, China. This revealed a community-wide preference for aspartate and a tight coupling between aspartate incorporation into DNA and the proliferation of aspartate utilizers during labeling. Both 13C incorporation into DNA and changes in the abundance of taxa during incubations indicated strong resource partitioning and a significant phylogenetic signal for aspartate incorporation. Of the active amplicon sequence variants (ASVs) identified by qSIP, most could be matched with genomes from Gongxiaoshe Hot Spring or nearby springs with an average nucleotide similarity of 99.4%. Genomes corresponding to aspartate primary utilizers were smaller, near-universally encoded polar amino acid ABC transporters, and had codon preferences indicative of faster growth rates. The most active ASVs assimilating both substrates were not abundant, suggesting an important role for the rare biosphere in the community response to organic carbon addition. The broad incorporation of aspartate into DNA over acetate by the hot spring community may reflect dynamic cycling of cell lysis products in situ or substrates delivered during monsoon rains and may reflect N limitation.
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影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
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影响因子：
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作者：
Briggs, Brandon R.;Brodie, Eoin L.;Hungate, Bruce A.
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Hungate, Bruce A.
影响因子：
15.5
作者：
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影响因子：
4.4
作者：
Coskun ÖK;Pichler M;Vargas S;Gilder S;Orsi WD
通讯作者：
Orsi WD
影响因子：
2.5
作者：
AZAM, F;FENCHEL, T;THINGSTAD, F
通讯作者：
THINGSTAD, F