The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer.
The clinical significance of DC-SIGN and DC-SIGNR, which are novel markers expressed in human colon cancer.
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DOI：
10.1371/journal.pone.0114748
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zuo Y
中科院分区：
文献类型：
--
作者：
Jiang Y;Zhang C;Chen K;Chen Z;Sun Z;Zhang Z;Ding D;Ren S;Zuo Y
结肠癌总是在晚期才被诊断出来，这与预后差有关。目前使用的血清肿瘤标志物CEA和CA19-9敏感性和特异性较低，可能对早期结肠癌没有诊断价值。因此，迫切需要寻找新的血清生物标志物用于结肠癌的早期检测。本研究采用酶联免疫吸附试验（ELISA）检测DC-SIGN和DC-SIGNR在血清中的表达。免疫组化（IHC）检测DC-SIGN和DC-SIGNR在癌组织中的表达。患者sDC-SIGNR水平低于健康对照组，而患者sDC-SIGNR水平高于健康对照组。sDC-SIGN和sDC-SIGNR对癌症患者均有诊断意义，两者联合使用的诊断价值高于单独使用。此外，在I/II期患者和健康对照组中，sDC-SIGN和sDC-SIGNR存在显著差异。sDC-SIGN水平高，存活时间长。此外，DC-SIGNR在癌灶和正常结肠组织中呈阴性，但在癌灶之间呈弱阳性。DC-SIGN染色在配对的正常结肠组织中较弱，在肿瘤间质和结肠癌组织浸润缘中较强，与同一患者血清中DC-SIGN水平呈负相关。有趣的是，DC-SIGN平均密度为>0.001219（匹配正常结肠组织的95%置信区间上限）的患者的生存率高于所有其他患者。DC-SIGN和DC-SIGNR是基于血液的分子标记物，可用于早期患者的诊断。此外，DC-SIGN在血清和癌组织中的表达可能影响结肠癌患者的生存时间。
Colon cancer has always been diagnosed at a late stage, which is associated with poor prognosis. The currently used serum tumor markers CEA and CA19-9 display low sensitivity and specificity and may not have diagnostic value in early stage colon cancer. Thus, there is an urgent need to identify novel serum biomarkers for use in the early detection of colon cancer. In this study, the expression of DC-SIGN and DC-SIGNR in serum was detected by enzyme-linked immunosorbent assay (ELISA). DC-SIGN and DC-SIGNR expression was detected in cancer tissues by immunohistochemistry (IHC). The level of sDC-SIGN was lower in patients than in the healthy controls, while the level of sDC-SIGNR in patients was higher than in the healthy controls. Both sDC-SIGN and sDC-SIGNR had diagnostic significances for cancer patients, and the combined diagnosis of these two markers was higher than both of them alone. Furthermore, there were significant differences between both sDC-SIGN and sDC-SIGNR in stage I/II patients and the healthy controls. Moreover, high sDC-SIGN level was accompanied with the long survival time. Additionally, DC-SIGNR was negative in the cancer foci and matched normal colon tissues but was weakly positive between the cancer foci. DC-SIGN staining was faint in matched normal colon tissues, strong in the tumor stroma and the invasive margin of colon cancer tissues, and negatively correlated with the sDC-SIGN level in serum from the same patient. Interestingly, the percent survival of patients with a DC-SIGN mean density of>0.001219 (the upper 95% confidence interval of matched normal colon tissues) was higher than for all other patients. DC-SIGN and DC-SIGNR are blood-based molecular markers that can potentially be used for the diagnosis of early stage patients. Moreover, expression of DC-SIGN in serum and cancer tissues may affect the survival time for colon cancer patients.
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影响因子：
4.3
作者：
Meyer, Sandra;Tefsen, Boris;van Die, Irma
通讯作者：
van Die, Irma
影响因子：
20.3
作者：
Engering, A;van Vliet, SJ;van Kooyk, Y
通讯作者：
van Kooyk, Y
影响因子：
2.6
作者：
Tatangelo, Fabiana;Cantile, Monica;Botti, Gerardo
通讯作者：
Botti, Gerardo
影响因子：
7.4
作者：
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通讯作者：
Loddenkemper, Christoph
影响因子：
6.2
作者：
Ferroni, Patrizia;Roselli, Mario;Guadagni, Fiorella
通讯作者：
Guadagni, Fiorella