Dissecting the labdane-related diterpenoid biosynthetic gene clusters in rice reveals directional cross-cluster phytotoxicity.
Dissecting the labdane-related diterpenoid biosynthetic gene clusters in rice reveals directional cross-cluster phytotoxicity.
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DOI：
10.1111/nph.17806
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Yang B
中科院分区：
文献类型：
--
作者：
Li R;Zhang J;Li Z;Peters RJ;Yang B
水稻是一种主要粮食作物，是一种典型的粮食作物。它包含两个生物合成基因簇(BGC)，用于产生与拉布丹相关的二萜类化合物(LRD)，这些二萜类化合物在对抗生物和非生物胁迫中发挥重要作用。虽然植物BGCs一直受到遗传分析的影响，但这些分析在很大程度上局限于单基因的研究。CRISPR/Cas9介导的基因组编辑被用来精确地移除这些BGC中的每一个，以及同时敲除两个BGC。与植甲素生物合成相关的2号染色体(C2bgc)上的BGC缺失，而与吗丁内酯生物合成相关的4号染色体(C4bgc)上的bgc缺失，导致了一种类似病损的表型。这种表型依赖于c2BGC中编码细胞色素P450(CYP)单加氧酶的两个密切相关的基因：CYP76M7和CYP76M8。然而，CYP76M7不是多余的，而是与植物卡桑的产生有关，而CYP76M8与丁香内酯的生物合成有关。耐人寻味的是，两个BGC缺失的情况下，病变的模拟表型并不一致。这些结果揭示了定向的跨簇植物毒性，推测是由于在没有CYP76M7和CYP76M8的情况下，依赖于C4BGC的LRD中间体的积累，进一步突出了它们相互依赖的进化和驱动BGC组装的选择压力。
Rice (Oryza sativa) is a staple food crop and serves as a model cereal plant. It contains two biosynthetic gene clusters (BGCs) for the production of labdane‐related diterpenoids (LRDs), which serve important roles in combating biotic and abiotic stress. While plant BGCs have been subject to genetic analyses, these analyses have been largely confined to the investigation of single genes. CRISPR/Cas9‐mediated genome editing was used to precisely remove each of these BGCs, as well as simultaneously knock out both BGCs. Deletion of the BGC from chromosome 2 (c2BGC), which is associated with phytocassane biosynthesis, but not that from chromosome 4 (c4BGC), which is associated with momilactone biosynthesis, led to a lesion mimic phenotype. This phenotype is dependent on two closely related genes encoding cytochrome P450 (CYP) mono‐oxygenases, CYP76M7 and CYP76M8, from the c2BGC. However, rather than being redundant, CYP76M7 has been associated with the production of phytocassanes, whereas CYP76M8 is associated with momilactone biosynthesis. Intriguingly, the lesion mimic phenotype is not present in a line with both BGCs deleted. These results reveal directional cross‐cluster phytotoxicity, presumably arising from the accumulation of LRD intermediates dependent on the c4BGC in the absence of CYP76M7 and CYP76M8, further highlighting their interdependent evolution and the selective pressures driving BGC assembly.
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DOI：
10.1073/pnas.0701742104
发表时间：
2007-06-19
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Peters, Reuben J.
影响因子：
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作者：
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