Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues.
Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues.
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DOI：
10.1371/journal.pone.0003922
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Mao JJ
中科院分区：
文献类型：
--
作者：
Moioli EK;Clark PA;Chen M;Dennis JE;Erickson HP;Gerson SL;Mao JJ
Poor angiogenesis is a major road block for tissue repair. The regeneration of virtually all tissues is limited by angiogenesis, given the diffusion of nutrients, oxygen, and waste products is limited to a few hundred micrometers. We postulated that co-transplantation of hematopoietic and mesenchymal stem/progenitor cells improves angiogenesis of tissue repair and hence the outcome of regeneration. In this study, we tested this hypothesis by using bone as a model whose regeneration is impaired unless it is vascularized. Hematopoietic stem/progenitor cells (HSCs) and mesenchymal stem/progenitor cells (MSCs) were isolated from each of three healthy human bone marrow samples and reconstituted in a porous scaffold. MSCs were seeded in micropores of 3D calcium phosphate (CP) scaffolds, followed by infusion of gel-suspended CD34+ hematopoietic cells. Co-transplantation of CD34+ HSCs and CD34− MSCs in microporous CP scaffolds subcutaneously in the dorsum of immunocompromized mice yielded vascularized tissue. The average vascular number of co-transplanted CD34+ and MSC scaffolds was substantially greater than MSC transplantation alone. Human osteocalcin was expressed in the micropores of CP scaffolds and was significantly increased upon co-transplantation of MSCs and CD34+ cells. Human nuclear staining revealed the engraftment of transplanted human cells in vascular endothelium upon co-transplantation of MSCs and CD34+ cells. Based on additional in vitro results of endothelial differentiation of CD34+ cells by vascular endothelial growth factor (VEGF), we adsorbed VEGF with co-transplanted CD34+ and MSCs in the microporous CP scaffolds in vivo, and discovered that vascular number and diameter further increased, likely owing to the promotion of endothelial differentiation of CD34+ cells by VEGF. Together, co-transplantation of hematopoietic and mesenchymal stem/progenitor cells may improve the regeneration of vascular dependent tissues such as bone, adipose, muscle and dermal grafts, and may have implications in the regeneration of internal organs.
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影响因子：
--
作者：
Johnson, Peter C.;Mikos, Antonios G.;Jansen, John A.
通讯作者：
Jansen, John A.
影响因子：
4.8
作者：
Clark, Paul A.;Moioli, Eduardo K.;Mao, Jeremy J.
通讯作者：
Mao, Jeremy J.
影响因子：
1.7
作者：
Jyotsna, Maddury;Vemuganti, Geeta K.;Chandra, K. Sarat
通讯作者：
Chandra, K. Sarat
影响因子：
4.6
作者：
Fuchs, JR;Nasseri, BA;Vacanti, JP
通讯作者：
Vacanti, JP
影响因子：
3
作者：
Koike, Y;Uzuki, M;Sawai, T
通讯作者：
Sawai, T