Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd.
Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd.
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DOI：
10.4142/jvs.2014.15.3.361
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发表时间：
2014
影响因子：
1.8
通讯作者：
Yamaguchi R
中科院分区：
文献类型：
--
作者：
Trang NT;Hirai T;Yamamoto T;Matsuda M;Okumura N;Giang NT;Lan NT;Yamaguchi R
本研究的目的是评估猪繁殖与呼吸综合征病毒(PRRSV)在自然感染的猪体内的解剖定位，并确定是否可以用口服液检测感染动物的病毒。本研究包括2头母猪、7头2月龄生长猪和70头6月龄母猪。用套式逆转录聚合酶链式反应(NRT-PCR)检测血清和口腔液中的PRRSV，同时对肺、扁桃体和口腔相关组织进行NRT-PCR、免疫组织化学和原位杂交。在母猪的口腔液和扁桃体中发现了PRRSV。在所有7头生长猪的口腔液、扁桃体、唾液腺、口腔粘膜和肺中也检测到PRRSV。然而，只在两头生长猪中观察到病毒血症。双重染色显示PRRSV分布于扁桃体隐窝上皮内及邻近的巨噬细胞内。在母猪中，在将这些动物引入农场3-8周后检测到北美型PRRSV野毒株。这些结果证实了先前的发现，即PRRSV主要在扁桃体中复制，然后被排入口服液。因此，口服液采样可能是监测繁殖牛群中PRRSV的有效方法。
The objectives of the present study were to evaluate the anatomic localization of porcine reproductive and respiratory syndrome virus (PRRSV) in naturally infected pigs and to determine whether oral fluid could be used to detect the virus in infected animals. Two sows, seven 2-month-old grower pigs, and 70 6-month-old gilts were included in this study. PRRSV in sera and oral fluid were identified by nested reverse transcription PCR (nRT-PCR) while lung, tonsil, and tissue associated with oral cavity were subjected to nRT-PCR, immunohistochemistry, and in situ hybridization. In sows, PRRSV was identified in oral fluid and tonsils. PRRSV was also detected in oral fluid, tonsils, salivary glands, oral mucosa, and lungs of all seven grower pigs. However, viremia was observed in only two grower pigs. Double staining revealed that PRRSV was distributed in macrophages within and adjacent to the tonsillar crypt epithelium. In gilts, the North American type PRRSV field strain was detected 3 to 8 weeks after introducing these animals onto the farm. These results confirm previous findings that PRRSV primarily replicates in tonsils and is then shed into oral fluid. Therefore, oral fluid sampling may be effective for the surveillance of PRRSV in breeding herds.
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影响因子：
3.3
作者：
Rowland RR;Lawson S;Rossow K;Benfield DA
通讯作者：
Benfield DA
影响因子：
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作者：
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作者：
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Nauwynck, Hans J.