Lymphoid tissue tropism of porcine reproductive and respiratory syndrome virus replication during persistent infection of pigs originally exposed to virus in utero.
Lymphoid tissue tropism of porcine reproductive and respiratory syndrome virus replication during persistent infection of pigs originally exposed to virus in utero.
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DOI：
10.1016/j.vetmic.2003.07.006
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发表时间：
2003-10-30
影响因子：
3.3
通讯作者：
Benfield DA
中科院分区：
文献类型：
--
作者：
Rowland RR;Lawson S;Rossow K;Benfield DA
猪繁殖与呼吸综合征病毒(PRRSV)持续感染的能力是该疾病在世界范围内传播的主要因素。一些研究已经记录了出生后感染猪的病毒感染过程；然而，关于在宫内暴露于PRRSV的猪持续感染期间病毒复制的位置知之甚少。本研究对用PRRSV分离株VR-2332感染妊娠90d的三头母猪的病毒复制和PRRSV特异性抗体进行了数百天的跟踪观察。84%的猪出生时是病毒携带者，出生后21d内死亡率为54%。在大约60天时，通过病毒分离，猪的血清病毒呈阴性。对63-132日龄随机处死的猪的组织中病毒复制的分析表明，在肺和其他非淋巴器官中没有病毒的证据。然而，病毒很容易从扁桃体和淋巴结中回收，原位杂交鉴定这些组织是病毒复制的部位。即使复制水平较低，病毒也很容易传播给哨兵猪。到260天时，猪的血清呈阴性，不会将病毒传播给哨兵猪。300天后，剩下的猪被宰杀，血液和组织中没有病毒的证据。这项研究表明，先天性PRRSV感染的猪可以在很长一段时间内支持病毒复制，在此期间，病毒复制主要限于扁桃体和淋巴结。
The ability of porcine reproductive and respiratory syndrome virus (PRRSV) to establish a persistent infection is the principal contributing factor to the world-wide spread of the disease. Several studies have documented the course of viral infection in postnatally infected pigs; however, very little is known regarding sites of virus replication during persistent infection of pigs exposed to PRRSV in utero. In this study, virus replication and PRRSV-specific antibody were followed for several hundred days in a group of pigs derived from three sows infected at 90 days of gestation with PRRSV isolate VR-2332. Eighty-four percent of pigs were born viremic with a mortality of 54% within 21 days after birth. At approximately 60 days sera from pigs were negative for virus by virus isolation. Analysis of virus replication in the tissues of pigs randomly sacrificed between 63 and 132 days showed no evidence of virus in lung and other non-lymphoid organs. However, virus was easily recovered from tonsil and lymph nodes and in situ hybridization identified these tissues as sites of virus replication. Even though replication was at a low level, virus was easily transmitted to sentinel pigs. By 260 days pigs became seronegative and did not transmit virus to sentinel pigs. Sacrifice of remaining pigs after 300 days showed no evidence of virus in blood and tissues. This study shows that congenital PRRSV-infected pigs can support virus replication for an extended period during which virus replication is primarily restricted to tonsil and lymph nodes.
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