Field-Effect Transistor Biosensor for Rapid Detection of Ebola Antigen.
Field-Effect Transistor Biosensor for Rapid Detection of Ebola Antigen.
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DOI：
10.1038/s41598-017-11387-7
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发表时间：
2017-09-08
影响因子：
4.6
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Chen Y;Ren R;Pu H;Guo X;Chang J;Zhou G;Mao S;Kron M;Chen J
埃博拉病毒传播一种高度传染性的、经常致命的人类疾病，目前尚无特异性抗病毒治疗方法。因此，快速、准确和早期诊断埃博拉病毒病（EVD）对于公共卫生遏制工作至关重要，特别是在资源匮乏且EVD流行的发展中国家。我们开发了一种基于还原氧化石墨烯的场效应晶体管方法，用于埃博拉病毒抗原的实时检测。该方法利用石墨烯基材料的有吸引力的半导体特性，并立即产生埃博拉糖蛋白的高度灵敏和特异性检测。使用悬浮在稀释的PBS缓冲液、人血清和血浆中的埃博拉糖蛋白评估该方法在床旁技术中临床应用的可行性。这些结果表明，一个有前途的场效应晶体管生物传感器的EVD诊断的成功制造。
The Ebola virus transmits a highly contagious, frequently fatal human disease for which there is no specific antiviral treatment. Therefore, rapid, accurate, and early diagnosis of Ebola virus disease (EVD) is critical to public health containment efforts, particularly in developing countries where resources are few and EVD is endemic. We have developed a reduced graphene oxide-based field-effect transistor method for real-time detection of the Ebola virus antigen. This method uses the attractive semiconductor characteristics of graphene-based material, and instantaneously yields highly sensitive and specific detection of Ebola glycoprotein. The feasibility of this method for clinical application in point-of-care technology is evaluated using Ebola glycoprotein suspended in diluted PBS buffer, human serum, and plasma. These results demonstrate the successful fabrication of a promising field-effect transistor biosensor for EVD diagnosis.
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DOI：
10.1056/nejmoa1411100
发表时间：
2014-10-16
期刊：
The New England journal of medicine
影响因子：
--
作者：
WHO Ebola Response Team;Aylward B;Barboza P;Bawo L;Bertherat E;Bilivogui P;Blake I;Brennan R;Briand S;Chakauya JM;Chitala K;Conteh RM;Cori A;Croisier A;Dangou JM;Diallo B;Donnelly CA;Dye C;Eckmanns T;Ferguson NM;Formenty P;Fuhrer C;Fukuda K;Garske T;Gasasira A;Gbanyan S;Graaff P;Heleze E;Jambai A;Jombart T;Kasolo F;Kadiobo AM;Keita S;Kertesz D;Koné M;Lane C;Markoff J;Massaquoi M;Mills H;Mulba JM;Musa E;Myhre J;Nasidi A;Nilles E;Nouvellet P;Nshimirimana D;Nuttall I;Nyenswah T;Olu O;Pendergast S;Perea W;Polonsky J;Riley S;Ronveaux O;Sakoba K;Santhana Gopala Krishnan R;Senga M;Shuaib F;Van Kerkhove MD;Vaz R;Wijekoon Kannangarage N;Yoti Z
通讯作者：
Yoti Z
影响因子：
168.9
作者：
Broadhurst, Mara Jana;Kelly, John Daniel;Pollock, Nira R.
通讯作者：
Pollock, Nira R.
DOI：
10.1126/science.1259657
发表时间：
2014-09-12
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gire SK;Goba A;Andersen KG;Sealfon RS;Park DJ;Kanneh L;Jalloh S;Momoh M;Fullah M;Dudas G;Wohl S;Moses LM;Yozwiak NL;Winnicki S;Matranga CB;Malboeuf CM;Qu J;Gladden AD;Schaffner SF;Yang X;Jiang PP;Nekoui M;Colubri A;Coomber MR;Fonnie M;Moigboi A;Gbakie M;Kamara FK;Tucker V;Konuwa E;Saffa S;Sellu J;Jalloh AA;Kovoma A;Koninga J;Mustapha I;Kargbo K;Foday M;Yillah M;Kanneh F;Robert W;Massally JL;Chapman SB;Bochicchio J;Murphy C;Nusbaum C;Young S;Birren BW;Grant DS;Scheiffelin JS;Lander ES;Happi C;Gevao SM;Gnirke A;Rambaut A;Garry RF;Khan SH;Sabeti PC
通讯作者：
Sabeti PC
影响因子：
16.6
作者：
Cui S;Pu H;Wells SA;Wen Z;Mao S;Chang J;Hersam MC;Chen J
通讯作者：
Chen J
影响因子：
4.6
作者：
Chu CH;Sarangadharan I;Regmi A;Chen YW;Hsu CP;Chang WH;Lee GY;Chyi JI;Chen CC;Shiesh SC;Lee GB;Wang YL
通讯作者：
Wang YL