Tyrosine hydroxylase is required for cuticle sclerotization and pigmentation in Tribolium castaneum.
Tyrosine hydroxylase is required for cuticle sclerotization and pigmentation in Tribolium castaneum.
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DOI：
10.1016/j.ibmb.2010.01.004
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发表时间：
2010-03
影响因子：
3.8
通讯作者：
Arakane, Yasuyuki
中科院分区：
文献类型：
--
作者：
Gorman, Maureen J.;Arakane, Yasuyuki
新合成的昆虫角质层是柔软和苍白的，但在几个小时或几天内变得更坚固（硬化），并且通常更深（着色）。硬化和色素沉着途径中的第一步是酪氨酸的羟基化以产生3，4-二羟基苯丙氨酸（DOPA）。酪氨酸羟化酶（TH）是已知的色素沉着过程中催化这一反应，但TH在硬化的作用还没有记录。本研究的目的是确定TH是否需要角质层硬化的红粉甲虫，赤拟谷盗。我们使用定量RT-PCR来验证TH表达发生在角质层晒黑的时间和免疫组化来确认TH在硬化角质层下的上皮细胞中表达。此外，我们发现TH功能的减少（由RNA干扰介导）导致角质层色素沉着减少，色素沉着和无色角质层的硬度降低。这些结果表明，TH在昆虫角质层的硬化以及棕色色素沉着中是必需的。
Newly synthesized insect cuticle is soft and pale but becomes stronger (sclerotized) and often darker (pigmented) over several hours or days. The first step in the sclerotization and pigmentation pathways is the hydroxylation of tyrosine to produce 3,4-dihydroxyphenylalanine (DOPA). Tyrosine hydroxylase (TH) is known to catalyze this reaction during pigmentation, but a role for TH in sclerotization has not been documented. The goal of this study was to determine whether TH is required for cuticle sclerotization in the red flour beetle, Tribolium castaneum. We used quantitative RT-PCR to verify that TH expression occurs at the time of cuticle tanning and immunohistochemistry to confirm that TH is expressed in the epithelial cells underlying sclerotized cuticle. In addition, we found that a reduction in TH function (mediated by RNA interference) resulted in a decrease in cuticle pigmentation and a decrease in the hardness of both pigmented and colorless cuticle. These results demonstrate a requirement for TH in sclerotization as well as brown pigmentation of insect cuticle.
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影响因子：
5.4
作者：
Ninomiya, Yosuke;Hayakawa, Yoichi
通讯作者：
Hayakawa, Yoichi
DOI：
10.1016/0020-1790(84)90063-5
发表时间：
1984-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
KRAMER, KJ;MORGAN, TD;LOOKHART, GL
通讯作者：
LOOKHART, GL
DOI：
10.1073/pnas.0504982102
发表时间：
2005-08-09
影响因子：
11.1
作者：
Arakane, Y;Muthukrishnan, S;Kramer, KJ
通讯作者：
Kramer, KJ
DOI：
10.1016/0020-1790(87)90139-9
发表时间：
1987-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
ROSELAND, CR;KRAMER, KJ;HOPKINS, TL
通讯作者：
HOPKINS, TL
影响因子：
9.2
作者：
True, JR;Edwards, KA;Carroll, SB
通讯作者：
Carroll, SB