In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds.
In vivo bone regeneration assessment of offset and gradient melt electrowritten (MEW) PCL scaffolds.
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DOI：
10.1186/s40824-020-00196-1
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发表时间：
2020
影响因子：
11.3
通讯作者：
Hamlet S
中科院分区：
文献类型：
--
作者：
Abbasi N;Lee RSB;Ivanovski S;Love RM;Hamlet S
基于生物材料的骨组织工程是克服残余骨体积减少的一个有前途的解决方案。先前已经证明，三维熔融电写聚己内酯（PCL）支架的梯度和偏移结构可以成功地引导成骨细胞向成骨谱系分化，从而导致矿化。因此，本研究的目的是评估具有这些不同结构的PCL支架的体内骨传导能力。将250 μm和500 μm、500 μm纤维偏移量为50%（偏移量为50.50）、三层梯度250 ~ 500 ~ 750 μm（梯度为250top）和750 ~ 500 ~ 250 μm（梯度为750top）五种不同的磷酸钙涂层熔融电写PCL孔径支架植入啮齿动物临界尺寸颅骨缺损。空白缺陷被用作控制。愈合4周和8周后，通过显微计算机断层扫描和免疫组织化学评估新骨。明显更多的新形成的骨头显示在grad。250top支架植入后8周。组织学检查也显示软组织被新形成的骨取代，并完全覆盖了grad。250年前支架。而在植入8周后，250 μm、偏移量为50.50的支架和空白颅骨缺损均未完全愈合。免疫组织化学分析显示，在两个时间点，所有支架组均存在成骨标志物的表达。矿化标志物骨钙素在小鼠中检测强度最高。250top和500 μm支架。此外，内皮标志物的表达表明，强健的血管生成参与了修复过程。这些结果表明，梯度孔径结构为骨再生提供了优越的条件。
Biomaterial-based bone tissue engineering represents a promising solution to overcome reduced residual bone volume. It has been previously demonstrated that gradient and offset architectures of three-dimensional melt electrowritten poly-caprolactone (PCL) scaffolds could successfully direct osteoblast cells differentiation toward an osteogenic lineage, resulting in mineralization. The aim of this study was therefore to evaluate the in vivo osteoconductive capacity of PCL scaffolds with these different architectures. Five different calcium phosphate (CaP) coated melt electrowritten PCL pore sized scaffolds: 250 μm and 500 μm, 500 μm with 50% fibre offset (offset.50.50), tri layer gradient 250–500-750 μm (grad.250top) and 750–500-250 μm (grad.750top) were implanted into rodent critical-sized calvarial defects. Empty defects were used as a control. After 4 and 8 weeks of healing, the new bone was assessed by micro-computed tomography and immunohistochemistry. Significantly more newly formed bone was shown in the grad.250top scaffold 8 weeks post-implantation. Histological investigation also showed that soft tissue was replaced with newly formed bone and fully covered the grad.250top scaffold. While, the bone healing did not happen completely in the 250 μm, offset.50.50 scaffolds and blank calvaria defects following 8 weeks of implantation. Immunohistochemical analysis showed the expression of osteogenic markers was present in all scaffold groups at both time points. The mineralization marker Osteocalcin was detected with the highest intensity in the grad.250top and 500 μm scaffolds. Moreover, the expression of the endothelial markers showed that robust angiogenesis was involved in the repair process. These results suggest that the gradient pore size structure provides superior conditions for bone regeneration.
骨髓增生异常综合征患者体外培养的成骨细胞数量和功能异常变化
影响因子：
2.9
作者：
Gao S;Wang H;Jiang H;Fu R;Yu H;Liu C;Tao J;Shao Z
通讯作者：
Shao Z