Abnormal changes in the quantity and function of osteoblasts cultured in vitro in patients with myelodysplastic syndrome.
Abnormal changes in the quantity and function of osteoblasts cultured in vitro in patients with myelodysplastic syndrome.
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骨髓增生异常综合征患者体外培养的成骨细胞数量和功能异常变化
DOI：
10.3892/ol.2018.9206
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发表时间：
2018-10
期刊：
影响因子：
2.9
通讯作者：
Shao Z
中科院分区：
文献类型：
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作者：
Gao S;Wang H;Jiang H;Fu R;Yu H;Liu C;Tao J;Shao Z
骨髓生态位的改变可导致骨髓增生异常综合征（MDS）的发生。成骨细胞作为骨髓生态位的重要组成部分，在MDS的发生发展中起着关键作用。本研究调查了成骨细胞的数量和功能，并通过体外试验检测了信号通路的变化及其与MDS患者进展的相关性。MDS患者的骨祖细胞（CD 34 +OCN+）和OCN+ CD 34 −Lin−成骨细胞的比例显著低于正常对照组。结果表明，MDS患者成骨细胞的数量和活性均低于正常人。骨髓增生异常综合征患者成骨细胞活性与病情严重程度相关。从高危和极高危MDS患者（WHO基于分类的预后评分系统评分3-6）体外培养的成骨细胞数量减少。体外培养的成骨细胞中T细胞免疫球蛋白和粘蛋白结构域3（TIM 3）和Jagged 1的水平也增加。这些结果表明，MDS患者成骨细胞异常改变，成骨细胞异常改变与MDS的严重程度有关。
Changes in bone marrow niches can lead to the occurrence of myelodysplastic syndrome (MDS). As an important part of the bone marrow niche, osteoblasts serve a key role in the progression of MDS. The present study investigated the quantity and function of osteoblasts and, through in vitro assays, detected changes in signaling pathways and the association with progression in MDS patients. The ratios of osteoprogenitors (CD34+OCN+) and OCN+CD34−Lin− osteoblasts in MDS patients were significantly less than those of normal controls. The results of this study demonstrated that the quantity and activity of osteoblasts in MDS patients were lower than those in normal controls. Furthermore the activity of osteoblasts in patients correlated with the severity of MDS. The quantity of osteoblasts cultured in vitro from high-risk and very high-risk MDS patients (WHO Classification-Based Prognostic Scoring System score 3–6) was decreased. The levels of T-cell immunoglobulin and mucin domain-containing 3 (TIM3) and Jagged 1 were also increased in the osteoblasts in vitro. These results indicated that osteoblasts are abnormally altered in MDS patients, and that there are associations between abnormal changes of osteoblasts and the severity of MDS.
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影响因子：
20.3
作者：
Vardiman, James W.;Thiele, Juergen;Bloomfield, Clara D.
通讯作者：
Bloomfield, Clara D.
影响因子：
16.8
作者：
Sakuishi K;Jayaraman P;Behar SM;Anderson AC;Kuchroo VK
通讯作者：
Kuchroo VK
影响因子：
3.5
作者：
Grochowski CM;Loomes KM;Spinner NB
通讯作者：
Spinner NB
影响因子：
11.4
作者：
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影响因子：
64.8
作者：
通讯作者：
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