Design, Analysis, and Implementation of a Novel Biochemical Pathway for Ethylene Glycol Production in Clostridium autoethanogenum.
Design, Analysis, and Implementation of a Novel Biochemical Pathway for Ethylene Glycol Production in Clostridium autoethanogenum.
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DOI：
10.1021/acssynbio.1c00624
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发表时间：
2022-05-20
影响因子：
4.7
通讯作者：
Islam, M. Ahsanul
中科院分区：
文献类型：
--
作者：
Bourgade, Barbara;Humphreys, Christopher M.;Millard, James;Minton, Nigel P.;Islam, M. Ahsanul
关键词：
平台化学品乙二醇（EG）用于制造具有工业重要性的各种商品化学品，但主要仍由化石燃料合成。虽然已经报道了几种新的代谢途径，其在模式生物的生物生产，没有被报道的气体发酵，非模式产乙酸底盘生物。在这里，我们描述了一种新的，合成的生化途径，将乙酸转化为EG在工业上重要的气体发酵产乙酸菌，梭菌autoethanogenum。我们不仅开发了一个计算工作流程来设计和分析数百种用于EG生产的新型生化途径，而且还在所选宿主中展示了成功的途径构建。使用双质粒系统实现EG生产以绕过不可行的表达水平和潜在的毒性酶相互作用。虽然只有0.029 g EG/g果糖的产量，因此需要进一步的菌株工程努力，以优化设计的菌株，这项工作证明了一个重要的概念验证的方法来计算设计和实验实施完全合成代谢途径的代谢高度特异性，非模型宿主生物体。
The platform chemical ethylene glycol (EG) is used to manufacture various commodity chemicals of industrial importance, but largely remains synthesized from fossil fuels. Although several novel metabolic pathways have been reported for its bioproduction in model organisms, none has been reported for gas-fermenting, non-model acetogenic chassis organisms. Here, we describe a novel, synthetic biochemical pathway to convert acetate into EG in the industrially important gas-fermenting acetogen,Clostridium autoethanogenum. We not only developed a computational workflow to design and analyze hundreds of novel biochemical pathways for EG production but also demonstrated a successful pathway construction in the chosen host. The EG production was achieved using a two-plasmid system to bypass unfeasible expression levels and potential toxic enzymatic interactions. Although only a yield of 0.029 g EG/g fructose was achieved and therefore requiring further strain engineering efforts to optimize the designed strain, this work demonstrates an important proof-of-concept approach to computationally design and experimentally implement fully synthetic metabolic pathways in a metabolically highly specific, non-model host organism.
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影响因子：
8.4
作者：
Liew F;Henstra AM;Kӧpke M;Winzer K;Simpson SD;Minton NP
通讯作者：
Minton NP
DOI：
10.1007/978-1-62703-299-5_9
发表时间：
2013-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Carbonell, Pablo;Planson, Anne-Gaelle;Faulon, Jean-Loup
通讯作者：
Faulon, Jean-Loup
通过（d） - 木糖通过大肠杆菌实施的合成途径优化乙二醇生产。
影响因子：
6.4
作者：
Alkim C;Cam Y;Trichez D;Auriol C;Spina L;Vax A;Bartolo F;Besse P;François JM;Walther T
通讯作者：
Walther T
影响因子：
14.9
作者：
Chou CH;Chang WC;Chiu CM;Huang CC;Huang HD
通讯作者：
Huang HD
影响因子：
4.4
作者：
Diner BA;Fan J;Scotcher MC;Wells DH;Whited GM
通讯作者：
Whited GM