Evaluation of candidate spermatogonial markers ID4 and GPR125 in testes of adult human cadaveric organ donors.
Evaluation of candidate spermatogonial markers ID4 and GPR125 in testes of adult human cadaveric organ donors.
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DOI：
10.1111/j.2047-2927.2014.00226.x
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发表时间：
2014-07
期刊：
影响因子：
4.5
通讯作者：
Seandel M
中科院分区：
文献类型：
--
作者：
Sachs C;Robinson BD;Andres Martin L;Webster T;Gilbert M;Lo HY;Rafii S;Ng CK;Seandel M
人类精原干细胞 (SSC) 的最佳标记尚不清楚。最近与小鼠和其他动物中的 SSC 相关的基因包括基本螺旋-环-螺旋转录因子 ID4 和孤儿 G 蛋白偶联受体 GPR125。虽然 ID4 和 GPR125 被认为是 SSC 的推定标记，但尚未评估它们在人体组织中的共表达情况。此外，分别表达 ID4 和 GPR125 的人类精原细胞群的大小和特征均未知。解决这些问题的一个主要障碍是能否从没有已知生殖健康问题的捐赠者那里获得健康的成人睾丸组织。为了克服这一障碍，我们采用了来自一组新的器官捐献者（n = 16；年龄 17-68 岁）的健康睾丸组织，他们正在进行死后临床器官获取。使用免疫标记，我们发现 ID4 和 GPR125 在部分重叠的精原细胞群体中表达，并且在正常成人睾丸中更广泛地表达。此外，我们发现 ID4 的表达在衰老过程中保持稳定。这些发现表明，ID4 和 GPR125 与其他假定的人类干细胞标记物结合起来，可以有效地识别年轻和老年捐赠者中以前未被识别的人类精原细胞亚群。
The optimal markers for human spermatogonial stem cells (SSCs) are not known. Among the genes recently linked to SSCs in mice and other animals are the basic helix-loop-helix transcription factor ID4 and the orphan G-protein coupled receptor GPR125. While ID4 and GPR125 are considered putative markers for SSCs, they have not been evaluated for co-expression in human tissue. Further, neither the size nor the character of the human spermatogonial populations that express ID4 and GPR125, respectively, are known. A major barrier to addressing these questions is the availability of healthy adult testis tissue from donors with no known reproductive health problems. To overcome this obstacle, we have employed healthy testicular tissue from a novel set of organ donors (n=16; aged 17-68 years) who were undergoing post-mortem clinical organ procurement. Using immunolabeling, we found that ID4 and GPR125 are expressed on partially overlapping spermatogonial populations and are more broadly expressed in the normal adult human testis. Additionally, we found that expression of ID4 remained stable during aging. These findings suggest that ID4 and GPR125 could be efficacious for identifying previously unrecognized human spermatogonial subpopulations in conjunction with other putative human stem cell markers, both in younger and older donors.
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影响因子：
64.5
作者：
Hobbs RM;Seandel M;Falciatori I;Rafii S;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
3.8
作者：
von Kopylow, Kathrein;Staege, Hannah;Kirchhoff, Christiane
通讯作者：
Kirchhoff, Christiane
影响因子：
3.6
作者：
He, Zuping;Kokkinaki, Maria;Dym, Martin
通讯作者：
Dym, Martin
影响因子：
64.5
作者：
BENEZRA, R;DAVIS, RL;WEINTRAUB, H
通讯作者：
WEINTRAUB, H
影响因子：
6.1
作者：
McLachlan, R. I.;Rajpert-De Meyts, E.;Skakkebaek, N. E.
通讯作者：
Skakkebaek, N. E.