Voltage-dependent activation and inactivation of calcium channels in PC12 cells. Correlation with neurotransmitter release.
Voltage-dependent activation and inactivation of calcium channels in PC12 cells. Correlation with neurotransmitter release.
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PC12 细胞中钙通道的电压依赖性激活和失活。
DOI：
10.1016/s0021-9258(18)48066-0
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Pozzan
中科院分区：
文献类型：
--
作者：
F. Di Virgilio;D. Milani;A. Leon;J. Meldolesi;T. Pozzan
The existence and mechanisms of inactivation of voltage-gated Ca2+ channels are important, but still debatable, physiological problems. By using the Ca2+ indicators quin2 and fura-2, we demonstrate that in PC12 cells voltage-gated Ca2+ channels undergo inactivation dependent on both voltage and [Ca2+]i. Inactivation, however, is never complete and a small number of channels remains open during prolonged depolarization, explaining the steady state elevation of [Ca2+]i observed in cells depolarized with high KCl. A close parallel exists between Ca2+ channel inactivation and the transient nature of neurotransmitter release: secretion is rapidly stimulated during the first 30 s of depolarization, when a transient overshoot in [Ca2+]i can be demonstrated, while it is negligible during the following period, despite the persistence of an elevated [Ca2+]i; predepolarization in Ca2+-free medium and subsequent addition of Ca2+ (a condition which allows the development of the voltage inactivation) abolishes the fast phase of secretion, while not modifying the steady state [Ca2+]i eventually attained; and increases in the intracellular Ca2+ buffering decreases the amplitude of the fast secretion phase induced by KCl without altering the steady state [Ca2+]i. We suggest that localized [Ca2+]i gradients form close to the plasma membrane shortly after depolarization and that the [Ca2+]i reached in these regions is the relevant parameter in the regulation of secretion.
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神经生长因子调节 PC-12 细胞释放神经递质的药物敏感性。
DOI：
10.1073/pnas.83.7.2243
发表时间：
1986
影响因子：
11.1
作者：
Kongsamut,S;Miller,RJ
通讯作者：
Miller,RJ
洋地黄皂苷通透的肾上腺髓质嗜铬细胞引起钙分泌。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wilson,SP;Kirshner,N
通讯作者：
Kirshner,N
DOI：
10.1126/science.6330896
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lux,HD;Brown,AM
通讯作者：
Brown,AM
DOI：
10.1016/s0021-9258(18)32526-2
发表时间：
1983-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Dunn;R. Holz
通讯作者：
L. Dunn;R. Holz
钙拮抗剂 高亲和力结合并抑制克隆细胞系中的钙转运。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Toll,L
通讯作者：
Toll,L