Paper-based pump-free magnetophoresis.
Paper-based pump-free magnetophoresis.
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DOI：
10.1039/d0ay01523g
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发表时间：
2020-11-21
期刊：
影响因子：
--
通讯作者：
Henry CS
中科院分区：
文献类型：
--
作者：
Call ZD;Carrell CS;Jang I;Geiss BJ;Dandy DS;Henry CS
微流控磁泳是一种强大的技术，用于从复杂的基质中分离和/或隔离感兴趣的细胞以进行分析。然而，需要机械泵来驱动流量，限制了便携性，并且使得转换为现场护理 (POC) 设置变得困难。微流控纸基分析装置 (μPAD) 提供了传统微流控装置的替代方案，不需要外部泵来产生流量。然而，μPAD 通常不用于颗粒分析，因为大多数颗粒会被困在多孔纤维网络中。在这里，我们报告了新开发的快速流动微流体纸基分析装置（ffPAD）执行磁泳的能力。 ffPAD 利用纸张堆叠层和透明片材之间的间隙中的毛细管作用来驱动比传统 μPAD 更高的流动速度。多层 ffPAD 允许颗粒和细胞穿过间隙，而不会被困在纸层中。我们首先证明 ffPAD 能够在带有钕永磁体的 μPAD 中实现磁性颗粒分离，并研究影响性能的关键因素。为了证明实用性，使用大肠杆菌作为模型分析物，并在用荧光标记抗体检测之前从人尿液中分离出来。人尿液中大肠杆菌标记磁珠的捕获效率为 61.5%。未来的研究将着眼于捕获效率的提高，并使该测定完全在芯片外进行，而不需要荧光标记。这里描述的测定和装置展示了基于纸的无泵微流体装置中磁泳的第一个例子。
Microfluidic magnetophoresis is a powerful technique that is used to separate and/or isolate cells of interest from complex matrices for analysis. However, mechanical pumps are required to drive flow, limiting portability and making translation to point-of-care (POC) settings difficult. Microfluidic paper-based analytical devices (μPADs) offer an alternative to traditional microfluidic devices that do not require external pumps to generate flow. However, μPADs are not typically used for particle analysis because most particles become trapped in the porous fiber network. Here we report the ability of newly developed fast-flow microfluidic paper-based analytical devices (ffPADs) to perform magnetophoresis. ffPADs use capillary action in a gap between stacked layers of paper and transparency sheets to drive flow at higher velocities than traditional μPADs. The multi-layer ffPADs allow particles and cells to move through the gap without being trapped in the paper layers. We first demonstrate that ffPADs enable magnetic particle separations in a μPAD with a neodymium permanent magnet and study key factors that affect performance. To demonstrate utility, E. coli was used as a model analyte and was isolated from human urine before detection with a fluorescently labeled antibody. A capture efficiency of 61.5% was then obtained of E. coli labeled magnetic beads in human urine. Future studies will look at the improvement of the capture efficiency and to make this assay completely off-chip without the need of a fluorescent label. The assay and device described here demonstrate the first example of magnetophoresis in a paper based, pump free microfluidic device.
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DOI：
10.1038/nrmicro3432
发表时间：
2015-05
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Flores-Mireles AL;Walker JN;Caparon M;Hultgren SJ
通讯作者：
Hultgren SJ
影响因子：
9.2
作者：
Choban, ER;Markoski, LJ;Kenis, PJA
通讯作者：
Kenis, PJA
影响因子：
9.4
作者：
Mahony, James B.;Blackhouse, Gord;Goeree, Ron
通讯作者：
Goeree, Ron
影响因子：
7.4
作者：
Martinez, Andres W.;Phillips, Scott T.;Carrilho, Emanuel
通讯作者：
Carrilho, Emanuel
影响因子：
6.1
作者：
Pamme, Nicole;Wilhelm, Claire
通讯作者：
Wilhelm, Claire