Rfc5p regulates alternate RFC complex functions in sister chromatid pairing reactions in budding yeast.
Rfc5p regulates alternate RFC complex functions in sister chromatid pairing reactions in budding yeast.
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DOI：
10.4161/cc.9.21.13634
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发表时间：
2010-11-01
期刊：
影响因子：
--
通讯作者：
Skibbens RV
中科院分区：
文献类型：
--
作者：
Maradeo ME;Garg A;Skibbens RV
姐妹染色单体配对反应，称为凝聚力的建立，发生在S期，似乎是由复制因子C（RFC）复合物调节。例如，含有Ctf 18 p的RFC表现出支持建立的活动，而含有Elg 1 p的RFC表现出反建立的活动。目前尚不清楚Ctf 18 p-RFC和Elg 1 p-RFC功能是否只是相反，还是揭示了复杂和非平行的调节机制。为了更好地理解这些新途径的性质，我们分析了Ctf 18 p-RFC和Elg 1 p-RFC共同的小RFC亚基Rfc 5 p。尽管存在这种共性，但数据显示Rfc 5 p功能的减弱挽救了ctf 7/eco 1突变细胞表型，揭示了Rfc 5 p促进抗建立活性。这种拯救对于建立途径是特异性的，因为rfc 5 -1在scc 2（沉积）、mcd 1/scc 1或smc 3（凝聚维持）突变细胞中表达时极大地加重了生长缺陷。我们的研究结果首次揭示了小RFC亚基在指导RFC复杂功能中的作用-在这种情况下，朝向反建立途径。我们进一步报告说，Pds 5 p表现出建立和反建立的凝聚力的功能。这种双重性表明，需要进一步审查建立和反建立活动的分类。
Sister chromatid pairing reactions, termed cohesion establishment, occur during S-phase and appear to be regulated by Replication Factor C (RFC) complexes. For instance, RFCs that contain Ctf18p exhibit pro-establishment activities while those that contain Elg1p exhibit anti-establishment activities. It remains unknown whether Ctf18p-RFC and Elg1p-RFC functions are simply opposing or instead reveal complicated and non-parallel regulatory mechanisms. To better understand the nature of these novel pathways, we analyzed the small RFC subunit Rfc5p that is common to both Ctf18p-RFC and Elg1p-RFC. Despite this commonality, the data show that diminished Rfc5p function rescues ctf7/eco1 mutant cell phenotypes, revealing that Rfc5p promotes anti-establishment activities. This rescue is specific to establishment pathways in that rfc5-1 greatly accentuates growth defects when expressed in scc2 (deposition), mcd1/scc1 or smc3 (cohesion maintenance) mutated cells. Our results reveal for the first time a role for small RFC subunits in directing RFC complex functions – in this case towards anti-establishment pathways. We further report that Pds5p exhibits both establishment and anti-establishment functions in cohesion. This duality suggests that categorizations of establishment and anti-establishment activities require further examination.
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DOI：
10.1073/pnas.1434308100
发表时间：
2003-09-02
影响因子：
11.1
作者：
Bermudez, VP;Maniwa, Y;Hurwitz, J
通讯作者：
Hurwitz, J
影响因子：
5.3
作者：
Naiki, T;Shimomura, T;Sugimoto, K
通讯作者：
Sugimoto, K
影响因子：
5.3
作者：
Shimomura, T;Ando, S;Sugimoto, K
通讯作者：
Sugimoto, K
DOI：
10.1083/jcb.151.3.613
发表时间：
2000-10-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hartman T;Stead K;Koshland D;Guacci V
通讯作者：
Guacci V
影响因子：
16
作者：
Lengronne, Armelle;McIntyre, John;Uhlmann, Frank
通讯作者：
Uhlmann, Frank