Pds5p is an essential chromosomal protein required for both sister chromatid cohesion and condensation in Saccharomyces cerevisiae.
Pds5p is an essential chromosomal protein required for both sister chromatid cohesion and condensation in Saccharomyces cerevisiae.
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DOI：
10.1083/jcb.151.3.613
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发表时间：
2000-10-30
期刊：
影响因子：
--
通讯作者：
Guacci V
中科院分区：
文献类型：
--
作者：
Hartman T;Stead K;Koshland D;Guacci V
PDS5基因（姐妹早熟分离）是在一项旨在鉴定对染色体结构重要的基因的遗传筛选中鉴定出来的。PDS5是一种必需基因，从酵母到人类都发现了其同源物。Pds5p功能对于从S期到有丝分裂的活力是重要的，并且在该细胞周期窗口期间定位于染色体，该细胞周期窗口包括存在姐妹染色单体凝聚的时间。Pds5p是保持在着丝粒近端和远端序列的凝聚力所必需的。这些性质与四个内聚复合物成员Mcd1p/Scc1p、Smc1p、Smc3p和Scc3p/Irr1p的性质相同（Guacci，V.，D. Koshland和A.斯特伦尼科夫1997. cell. 91：47 - 57; Michaelis，C.，R. Ciosk和K.内史密斯1997. cell. 91：35 - 45; Toth，A.，R. Ciosk，F.乌尔曼，M。Galova，A. Schleiffer和K.内史密斯1999. genes Dev. 13：307 - 319）。Pds5p与Mcd1p结合的着丝粒和臂序列结合。此外，Pds5p定位于染色体依赖于Mcd1p。因此，Pds5p，像cohesin复合体成员，是介导姐妹染色单体凝聚力的分子胶的一个组成部分。然而，Mcd1p定位到染色体是独立的Pds5p，这可能反映了他们的凝聚力的作用的差异。最后，Pds5p是缩合和内聚所必需的，这证实了通过Mcd1p的分析揭示的这些过程之间的联系（Guacci，V.，D. Koshland和A.斯特伦尼科夫1997. cell. 91：47 - 57）。因此，凝聚和凝聚之间的联系是酵母染色体的一般性质。
The PDS5 gene (precocious dissociation of sisters) was identified in a genetic screen designed to identify genes important for chromosome structure. PDS5 is an essential gene and homologues are found from yeast to humans. Pds5p function is important for viability from S phase through mitosis and localizes to chromosomes during this cell cycle window, which encompasses the times when sister chromatid cohesion exists. Pds5p is required to maintain cohesion at centromere proximal and distal sequences. These properties are identical to those of the four cohesion complex members Mcd1p/Scc1p, Smc1p, Smc3p, and Scc3p/Irr1p (Guacci, V., D. Koshland, and A. Strunnikov. 1997. Cell. 91:47–57; Michaelis, C., R. Ciosk, and K. Nasmyth. 1997. Cell. 91:35–45; Toth, A., R. Ciosk, F. Uhlmann, M. Galova, A. Schleiffer, and K. Nasmyth. 1999. Genes Dev. 13:307–319). Pds5p binds to centromeric and arm sequences bound by Mcd1p. Furthermore, Pds5p localization to chromosomes is dependent on Mcd1p. Thus, Pds5p, like the cohesin complex members, is a component of the molecular glue that mediates sister chromatid cohesion. However, Mcd1p localization to chromosomes is independent of Pds5p, which may reflect differences in their roles in cohesion. Finally, Pds5p is required for condensation as well as cohesion, which confirms the link between these processes revealed through analysis of Mcd1p (Guacci, V., D. Koshland, and A. Strunnikov. 1997. Cell. 91:47–57). Therefore, the link between cohesion and condensation is a general property of yeast chromosomes.
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影响因子：
3.3
作者：
Guacci, V;Hogan, E;Koshland, D
通讯作者：
Koshland, D
影响因子：
3.3
作者：
Lavoie, BD;Tuffo, KM;Holm, C
通讯作者：
Holm, C
影响因子：
64.5
作者：
Blat, Y;Kleckner, N
通讯作者：
Kleckner, N
影响因子：
64.5
作者：
Kimura, K;Hirano, T
通讯作者：
Hirano, T
影响因子：
64.5
作者：
CHUANG, PT;ALBERTSON, DG;MEYER, BJ
通讯作者：
MEYER, BJ