Spray Drying Is a Viable Technology for the Preservation of Recombinant Proteins in Microalgae.
Spray Drying Is a Viable Technology for the Preservation of Recombinant Proteins in Microalgae.
复制标题
DOI：
10.3390/microorganisms11020512
复制
发表时间：
2023-02-17
期刊：
影响因子：
4.5
通讯作者：
Parker, Brenda
中科院分区：
文献类型：
--
作者：
Vilatte, Anaelle;Spencer-Milnes, Xenia;Jackson, Harry Oliver;Purton, Saul;Parker, Brenda
关键词：
微藻是生产包封重组蛋白如疫苗的有前途的宿主生物。然而，生物工艺开发中的瓶颈，如干燥阶段，需要得到解决，以确保规模的可行性。在这项研究中，我们研究了喷雾干燥在微藻中生产重组疫苗的潜力。通过叶绿体工程创建了携带针对鲑鱼甲病毒的亚单位疫苗的莱茵衣藻（Chlamyeliumreinhardtii）的转基因株系。通过免疫印迹评估喷雾干燥后重组蛋白的完整性及其在-80 °C、+4 °C和室温下储存27个月后的稳定性。蛋白质经受喷雾干燥而没有显著损失。在+4 °C和室温下长期储存分别导致50%和92%的降解。优化喷雾干燥和储存条件应尽量减少降解，并有利于在正温度下短期储存。使用产量和生产率的数据，喷雾干燥和冷冻干燥为基础的生物工艺的经济性进行了比较。干燥阶段相当于总生产成本的41%。工艺优化、基因工程和新市场战略被确定为降低成本的潜在目标。总体而言，本研究成功地证明了喷雾干燥作为工业规模微藻重组蛋白生产的工艺选择的适用性。
Microalgae are promising host organisms for the production of encapsulated recombinant proteins such as vaccines. However, bottlenecks in bioprocess development, such as the drying stage, need to be addressed to ensure feasibility at scale. In this study, we investigated the potential of spray drying to produce a recombinant vaccine in microalgae. A transformant line of Chlamydomonas reinhardtii carrying a subunit vaccine against salmonid alphavirus was created via chloroplast engineering. The integrity of the recombinant protein after spray drying and its stability after 27 months storage at –80 °C, +4 °C and room temperature were assessed by immunoblotting. The protein withstood spray drying without significant losses. Long-term storage at +4 °C and room temperature resulted in 50% and 92% degradation, respectively. Optimizing spray drying and storage conditions should minimize degradation and favour short-term storage at positive temperatures. Using data on yield and productivity, the economics of spray drying- and freeze drying-based bioprocesses were compared. The drying stage corresponded to 41% of the total production cost. Process optimization, genetic engineering and new market strategies were identified as potential targets for cost reduction. Overall, this study successfully demonstrates the suitability of spray drying as a process option for recombinant protein production in microalgae at the industrial scale.
登录
查看更多内容
影响因子：
3.3
作者：
Cho, Seo Young;Kim, Hyoung Jin;Kim, Hong-Jin
通讯作者：
Kim, Hong-Jin
影响因子：
4.2
作者：
Charoonnart P;Purton S;Saksmerprome V
通讯作者：
Saksmerprome V
DOI：
10.1016/j.algal.2017.11.038
发表时间：
2018-04-01
影响因子：
5.1
作者：
Fasaei, F.;Bitter, J. H.;van Boxtel, A. J. B.
通讯作者：
van Boxtel, A. J. B.
影响因子：
4.4
作者：
Gregory, James A.;Topol, Aaron B.;Mayfield, Stephen
通讯作者：
Mayfield, Stephen
微藻作为免疫增强剂和重组疫苗用于水产养殖疾病预防和控制的评价
影响因子：
5.7
作者：
Ma K;Bao Q;Wu Y;Chen S;Zhao S;Wu H;Fan J
通讯作者：
Fan J