Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.
Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.
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小鼠白细胞DNA修复相关基因表达的辐射剂量效应
DOI：
10.12659/msm.881976
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发表时间：
2011-10
期刊：
影响因子：
--
通讯作者：
Min R
中科院分区：
文献类型：
--
作者：
Li MJ;Wang WW;Chen SW;Shen Q;Min R
本研究的目的是从DNA修复相关的基因表达谱中筛选用于生物剂量测定的分子生物标志物。小鼠接受60Coγ全身照射，剂量范围为0~8Gy.剂量率为0.80Gy.min。于照射后4、8、12、24、48h从受照小鼠外周血中提取RNA。采用实时定量聚合酶链式反应(RT-PCR)检测与DNA损伤修复相关的11个基因的转录变化。在所检测的11个基因中，CDKN1A(细胞周期蛋白依赖性激酶抑制物1A或p21，Cip1)和ATM(共济失调毛细血管扩张突变)的表达水平分别随着照射后不同时间剂量的增加而显著上调和下调。RAD50(Rad50同系物)、PLK3(Polo-like kinase3)、GADD45A(生长停滞和DNA损伤诱导，α)、DDB2(损伤特异性DNA结合蛋白2)、BBC3(BCL2结合组分3)和IER5(即刻早期反应5)基因表达水平在照射后观察到的较大剂量范围内经历了显著的振荡变化。其中三个基因在观察到的暴露剂量和辐射后时间范围内没有变化。本研究的结果补充了生物标志物数据库中的生物剂量学，将有助于构建合适的基因表达生物标志物系统来评估辐射剂量。
The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles. Mice were subjected to whole-body exposure with 60Co γ rays with a dose range of 0–8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR). Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times. RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2–8 Gy at post-exposure time points observed. Three of the genes were found not to change within the observed exposure dose and post-radiation time ranges. The results of this study add to the biodosimetry with biomarker data pool and will be helpful for constructing appropriate gene expression biomarker systems to evaluate radiation exposure doses.
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影响因子：
3.7
作者：
Meadows, Sarah K.;Dressman, Holly K.;Muramoto, Garrett G.;Himburg, Heather;Salter, Alice;Wei, ZhengZheng;Ginsburg, Geoff;Chao, Nelson J.;Nevins, Joseph R.;Chute, John P.
通讯作者：
Chute, John P.
影响因子：
3.4
作者：
Paul S;Barker CA;Turner HC;McLane A;Wolden SL;Amundson SA
通讯作者：
Amundson SA
影响因子：
3.4
作者：
Costes, SV;Boissière, A;Barcellos-Hoff, MH
通讯作者：
Barcellos-Hoff, MH
影响因子：
2.6
作者：
Kabacik, Sylwia;Mackay, Alan;Badie, Christophe
通讯作者：
Badie, Christophe
DOI：
10.1016/j.ijrobp.2004.04.060
发表时间：
2004-10-01
影响因子：
7
作者：
Khodarev, NN;Kataoka, Y;Grdina, DJ
通讯作者：
Grdina, DJ