Gene expression signatures of radiation response are specific, durable and accurate in mice and humans.
Gene expression signatures of radiation response are specific, durable and accurate in mice and humans.
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DOI：
10.1371/journal.pone.0001912
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发表时间：
2008-04-02
期刊：
影响因子：
3.7
通讯作者：
Chute, John P.
中科院分区：
文献类型：
--
作者：
Meadows, Sarah K.;Dressman, Holly K.;Muramoto, Garrett G.;Himburg, Heather;Salter, Alice;Wei, ZhengZheng;Ginsburg, Geoff;Chao, Nelson J.;Nevins, Joseph R.;Chute, John P.
以前的工作已经证明了外周血（PB）基因表达谱在检测疾病或环境暴露方面的潜力。我们试图确定环境暴露、电离辐射对PB基因表达谱的几个变量的影响，并确定辐射与其他遗传毒性应激的PB特征的特异性。基因型差异和PB采样时间均不会降低PB特征预测辐射暴露的准确性，但性别差异会影响最低水平（50 cGy）下PB特征预测辐射暴露的准确性。我们还生成了脓毒症的PB特征，发现辐射的PB特征和脓毒症的PB特征在区分受辐射动物和脓毒症动物时都是100%特异性的。我们还确定了辐射暴露和化疗的人类PB特征，区分了异质人群中放疗患者和化疗个体，准确率分别为90%和81%。我们得出的结论是，PB基因表达谱可以在小鼠和人类中被识别出来，在预测医疗状况方面是准确的，对每种情况都是特定的，并且随着时间的推移保持高度准确。
Previous work has demonstrated the potential for peripheral blood (PB) gene expression profiling for the detection of disease or environmental exposures. We have sought to determine the impact of several variables on the PB gene expression profile of an environmental exposure, ionizing radiation, and to determine the specificity of the PB signature of radiation versus other genotoxic stresses. Neither genotype differences nor the time of PB sampling caused any lessening of the accuracy of PB signatures to predict radiation exposure, but sex difference did influence the accuracy of the prediction of radiation exposure at the lowest level (50 cGy). A PB signature of sepsis was also generated and both the PB signature of radiation and the PB signature of sepsis were found to be 100% specific at distinguishing irradiated from septic animals. We also identified human PB signatures of radiation exposure and chemotherapy treatment which distinguished irradiated patients and chemotherapy-treated individuals within a heterogeneous population with accuracies of 90% and 81%, respectively. We conclude that PB gene expression profiles can be identified in mice and humans that are accurate in predicting medical conditions, are specific to each condition and remain highly accurate over time.
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影响因子：
64.8
作者：
Alizadeh, AA;Eisen, MB;Staudt, LM
通讯作者：
Staudt, LM
影响因子：
3.4
作者：
Cromheecke, M;Piers, BA;Hoekstra, HJ
通讯作者：
Hoekstra, HJ
影响因子：
8.3
作者：
Baird, Alison E.
通讯作者：
Baird, Alison E.
DOI：
10.1016/0027-5107(95)00044-j
发表时间：
1995-07-01
影响因子：
2.3
作者：
HIRAI, Y;KUSUNOKI, Y;AKIYAMA, M
通讯作者：
AKIYAMA, M
DOI：
10.1073/pnas.231625398
发表时间：
2002-01-22
影响因子：
11.1
作者：
Boldrick, JC;Alizadeh, AA;Relman, DA
通讯作者：
Relman, DA