Improved protocol for spawning and immunostaining embryos and juvenile stages of the ctenophore Mnemiopsis leidyi
Improved protocol for spawning and immunostaining embryos and juvenile stages of the ctenophore Mnemiopsis leidyi
复制标题
栉水母 Mnemiopsis leidyi 产卵和免疫染色胚胎和幼年阶段的改进方案
DOI:
10.1038/protex.2018.092
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发表时间:
2018
期刊:
影响因子:
--
通讯作者:
Martindale, Mark Q.
中科院分区:
文献类型:
--
作者:
Salinas-Saavedra, Miguel;Salinas-Saavedra, Miguel;Martindale, Mark Q.
Ctenophores are becoming of increasing interest to researchers interested in cellular and developmental evolutionary biology. One species, Mnemiopsis leidyi, has been one of the most heavily studied ctenophores due to its availability and because it was the first ctenophore to have its genome sequenced and made publicly available. However, their gelatinous and delicate consistency makes experimental handling challenging and time-consuming. Here we describe improvements for scheduling adult spawning and new handling techniques for further histological and molecular analyses. We hope that this contribution will establish an opportunity for other workers to improve these methods and allow a rapid increase in our understanding of ctenophore biology.
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影响因子:
2.9
作者:
J. Henry;M. Martindale
通讯作者:
M. Martindale
影响因子:
7.7
作者:
Fidler, Aaron L.;Darris, Carl E.;Hudson, Billy G.
通讯作者:
Hudson, Billy G.
DOI:
10.1101/pdb.prot5086
发表时间:
2008
期刊:
CSH protocols
影响因子:
--
作者:
K. Pang;M. Martindale
通讯作者:
M. Martindale
影响因子:
4
作者:
M. Martindale
通讯作者:
M. Martindale
影响因子:
--
作者:
E. Houliston;D. Carré;P. Chang;C. Sardet
通讯作者:
C. Sardet