Improved protocol for spawning and immunostaining embryos and juvenile stages of the ctenophore Mnemiopsis leidyi

Improved protocol for spawning and immunostaining embryos and juvenile stages of the ctenophore Mnemiopsis leidyi
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栉水母 Mnemiopsis leidyi 产卵和免疫染色胚胎和幼年阶段的改进方案

DOI:
10.1038/protex.2018.092
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发表时间:
2018
期刊:
Protocol Exchange
影响因子:
--
通讯作者:
Martindale, Mark Q.
Martindale, Mark Q.
中科院分区:
--
文献类型:
--
作者:
Salinas-Saavedra, Miguel;Salinas-Saavedra, Miguel;Martindale, Mark Q.

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栉水母越来越受到细胞和发育进化生物学研究人员的关注。其中一个物种,Mnemiopsis leidyi,是研究最多的栉水母之一,因为它的可用性,因为它是第一个栉水母有其基因组测序和公开。然而,它们的凝胶状和微妙的一致性使得实验处理具有挑战性和耗时。在这里,我们描述了改进调度成人产卵和新的处理技术,进一步的组织学和分子分析。我们希望,这一贡献将建立一个机会,为其他工作人员,以改善这些方法,并允许在我们的理解栉水母生物学的快速增长。
Ctenophores are becoming of increasing interest to researchers interested in cellular and developmental evolutionary biology. One species, Mnemiopsis leidyi, has been one of the most heavily studied ctenophores due to its availability and because it was the first ctenophore to have its genome sequenced and made publicly available. However, their gelatinous and delicate consistency makes experimental handling challenging and time-consuming. Here we describe improvements for scheduling adult spawning and new handling techniques for further histological and molecular analyses. We hope that this contribution will establish an opportunity for other workers to improve these methods and allow a rapid increase in our understanding of ctenophore biology.
基础后生动物栉水母 Mnemiopsis leidyi 中的诱导相互作用和胚胎等价群
DOI: --
发表时间: 2004
影响因子: 2.9
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DOI: 10.7554/elife.24176
发表时间: 2017-04-18
期刊: ELIFE
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栉水母整体抗体染色。
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