Characterization of Pdgfrb-Cre transgenic mice reveals reduction of ROSA26 reporter activity in remodeling arteries.
Characterization of Pdgfrb-Cre transgenic mice reveals reduction of ROSA26 reporter activity in remodeling arteries.
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DOI：
10.1002/dvg.20769
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发表时间：
2011-08
期刊：
影响因子：
1.5
通讯作者：
Lindner, Volkhard
中科院分区：
文献类型：
--
作者：
Cuttler, Anne S.;LeClair, Renee J.;Stohn, J. Patrizia;Wang, Qiaozeng;Sorenson, Christine M.;Liaw, Lucy;Lindner, Volkhard
为了调节重塑血管的血管壁细胞中的基因表达，我们产生并表征了在血小板衍生生长因子受体-β启动子控制下具有Cre重组酶的转基因小鼠系，称为Tg（Pdgfrb-Cre）35 Vli。将转基因小鼠与Gt（ROSA）26 Sortm 1 Sor品系杂交，并通过β-半乳糖苷酶活性检查Cre活化，将其与内源性Pdgfrb表达进行比较。此外，Pdgfrb-Cre小鼠用于驱动条件性myc标记的Cthrc 1转基因的表达。β-半乳糖苷酶活性与内源性Pdgfrb免疫反应性有很好的重叠。然而，由颈动脉结扎诱导的血管壁细胞的去分化揭示了ROSA 26报告活性和Pdgfrb启动子驱动的Cre依赖性myc标记的Cthrc 1转基因表达之间的显著差异。我们的研究证明了Pdgfrb-Cre小鼠驱动条件性转基因表达的能力，这是已知表达内源性Pdgfrb的组织中先前Cre介导的重组的结果。此外，该研究表明，ROSA 26启动子驱动的报告小鼠不适用于重塑血管中平滑肌的谱系标记。
With the intention to modulate gene expression in vascular mural cells of remodeling vessels, we generated and characterized transgenic mouse lines with Cre recombinase under the control of the platelet-derived growth factor receptor-β promoter, referred to as Tg(Pdgfrb-Cre)35Vli. Transgenic mice were crossed with the Gt(ROSA)26Sortm1Sor strain and examined for Cre activation by β-galactosidase activity, which was compared with endogenous Pdgfrb expression. In addition, Pdgfrb-Cre mice were used to drive expression of a conditional myc-tagged Cthrc1 transgene. There was good overlap of β-galactosidase activity with endogenous Pdgfrb immunoreactivity. However, dedifferentiation of vascular mural cells induced by carotid artery ligation revealed a dramatic discrepancy between ROSA26 reporter activity and Pdgfrb promoter driven Cre dependent myc-tagged Cthrc1 transgene expression. Our studies demonstrate the capability of the Pdgfrb-Cre mouse to drive conditional transgene expression as a result of prior Cre mediated recombination in tissues known to express endogenous Pdgfrb. In addition, the study shows that ROSA26 promoter driven reporter mice are not suitable for lineage marking of smooth muscle in remodeling blood vessels.
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影响因子：
10.5
作者：
SORIANO, P
通讯作者：
SORIANO, P
影响因子：
11.8
作者：
Yamamoto, Shinji;Nishimura, Osamu;Sasaki, Hiroshi
通讯作者：
Sasaki, Hiroshi
DOI：
10.1161/01.atv.17.10.2238
发表时间：
1997-10-01
影响因子：
8.7
作者：
Kumar, A;Lindner, V
通讯作者：
Lindner, V
影响因子：
19.6
作者：
ALPERS, CE;SEIFERT, RA;BOWENPOPE, DF
通讯作者：
BOWENPOPE, DF
影响因子：
15.9
作者：
Regan, CP;Adam, PJ;Owens, GK
通讯作者：
Owens, GK