Effects of ursolic and oleanolic on SK‑MEL‑2 melanoma cells: In vitro and in vivo assays.
Effects of ursolic and oleanolic on SK‑MEL‑2 melanoma cells: In vitro and in vivo assays.
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DOI：
10.3892/ijo.2017.4160
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发表时间：
2017-12
影响因子：
5.2
通讯作者：
Dehelean C
中科院分区：
文献类型：
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作者：
Caunii A;Oprean C;Cristea M;Ivan A;Danciu C;Tatu C;Paunescu V;Marti D;Tzanakakis G;Spandidos DA;Tsatsakis A;Susan R;Soica C;Avram S;Dehelean C
在三萜类化合物中，油酸（OA）及其异构体熊果酸（UA）是有前途的治疗候选物，在黑色素瘤的管理中具有潜在的益处。在这项研究中，我们的目的是检查在体外和体内的抗侵袭和抗转移活性的OA和UA，以确定其可能的有用性作为化学预防或化疗药物在黑色素瘤。对于体外实验，检查三萜化合物对SK-MEL-2黑素瘤细胞的抗增殖活性。通过测试活性化合物对血管细胞粘附分子（VCAM）和细胞间粘附分子（ICAM）粘附至黑色素瘤细胞的影响来评估抗侵袭潜力。采用鸡胚绒毛尿囊膜（CAM）法检测正常和肿瘤血管生成。两种测试三萜酸，UA和OA，在体外和体内对SK-MEL-2黑色素瘤细胞产生不同的作用。与OA相比，UA在体外发挥了显著的剂量依赖性抗增殖作用。体外对黑色素瘤细胞的细胞毒性作用通过检查UA诱导的细胞周期阶段的改变来确定，所述改变导致细胞停滞在S期。此外，发现UA通过限制细胞对ICAM分子的粘附能力而影响SK-MEL-2黑色素瘤细胞的侵袭性，但不影响它们对VCAM分子的粘附。总体而言，在本研究中，通过评估两种三萜类化合物在体内的作用，我们的结果显示，OA具有更大的潜力，削弱侵袭能力和肿瘤血管生成相比，UA。
Among the triterpenoids, oleanolic acid (OA) and its isomer, ursolic acid (UA) are promising therapeutic candidates, with potential benefits in the management of melanoma. In this study, we aimed to examine the in vitro and in vivo anti-invasive and anti-metastatic activity of OA and UA to determine their possible usefulness as chemopreventive or chemotherapeutic agents in melanoma. For the in vitro experiments, the anti-proliferative activity of the triterpenic compounds on SK-MEL-2 melanoma cells was examined. The anti-invasive potential was assessed by testing the effects of the active compound on vascular cell adhesion molecule (VCAM) and intercellular adhesion molecule (ICAM) adhesion to melanoma cells. Normal and tumor angiogenesis were evaluated in vivo by chicken embryo chorioallantoic membrane (CAM) assay. The two test triterpenoid acids, UA and OA, exerted differential effects in vitro and in vivo on the SK-MEL-2 melanoma cells. UA exerted a significant and dose-dependent anti-proliferative effect in vitro, compared to OA. The cytotoxic effects in vitro on the melanoma cells were determined by the examining alterations in the cell cycle phases induced by UA that lead to cell arrest in the S phase. Moreover, UA was found to affect SK-MEL-2 melanoma cell invasiveness by limiting the cell adhesion capacity to ICAM molecules, but not influencing their adhesion to VCAM molecules. On the whole, in this study, by assessing the effects of the two triterpenoids in vivo, our results revealed that OA had a greater potential to impair the invasive capacity and tumor angiogenesis compared with UA.
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影响因子：
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作者：
Bendas G;Borsig L
通讯作者：
Borsig L
影响因子：
2.8
作者：
Chen, Chong;Zhang, Qiuyang;Liu, Sen;Parajuli, Keshab R.;Qu, Yine;Mei, Jiandong;Chen, Zhiquan;Zhang, Hui;Khismatullin, Damir B.;You, Zongbing
通讯作者：
You, Zongbing
影响因子：
5.6
作者：
Avram, Sorin I.;Pacureanu, Liliana M.;Kurunczi, Ludovic
通讯作者：
Kurunczi, Ludovic
影响因子：
4.3
作者：
Johnson, Douglas B.;Sosman, Jeffrey A.
通讯作者：
Sosman, Jeffrey A.
DOI：
10.3390/molecules22030400
发表时间：
2017-03-04
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
J C Furtado NA;Pirson L;Edelberg H;M Miranda L;Loira-Pastoriza C;Preat V;Larondelle Y;André CM
通讯作者：
André CM