Determination of tripdiolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reversed-phase high-performance liquid chromatography.
Determination of tripdiolide in root extracts of Tripterygium wilfordii by solid-phase extraction and reversed-phase high-performance liquid chromatography.
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DOI：
10.1002/pca.1059
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发表时间：
2008-07
影响因子：
3.3
通讯作者：
Raskin, Ilya
中科院分区：
文献类型：
--
作者：
Ma, Jun;Schmidt, Barbara M.;Poulev, Alexander;Raskin, Ilya
雷公藤提取物在中国已广泛用于治疗多种自身免疫性疾病和炎症性疾病。二萜类化合物雷公藤甲内酯和雷公藤二内酯是雷公藤乙酸乙酯提取物中的两种主要活性成分。先前报道了一种有效的固相萃取和高效液相色谱（SPE-HPLC）方法来测量提取物中雷公藤甲素的含量。然而，由于提取物中存在与雷公藤内酯共洗脱的干扰化合物，因此没有合适的雷公藤内酯定量方法。因此，本文描述了一种可以从少量提取物中测量雷公藤内酯含量的方法。将提取液 (600 µL) 添加到氨丙基 SPE 管中。用二氯甲烷:甲醇(1 mL, 49:1 v/v)洗脱雷公藤内酯，然后用二氯甲烷:甲醇(3 mL, 17:3 v/v)洗脱雷公藤内酯。使用等度溶剂系统通过 HPLC 分析雷公藤内酯洗脱液，并通过测量 219 nm 处的峰面积进行定量。提取物中雷公藤甲内酯和雷公藤内酯的含量分别为807.32±51.94和366.13±17.21μg/g提取物。由于雷公藤内酯具有生物活性，并且在提取物中占相当大的比例，出于提取物质量控制和标准化目的，应使用建议的 SPE-HPLC 方法与雷公藤内酯一起进行测量。
Extracts of Tripterygium wilfordii Hook F. have been widely used in China to treat a variety of autoimmune and inflammatory diseases. The diterpenoids triptolide and tripdiolide are two major active components in the T. wilfordii ethyl acetate extract. An efficient solid-phase extraction and high-performance liquid chromatography (SPE-HPLC) method to measure triptolide content in the extract has been previously reported. However, a suitable means of tripdiolide quantification is not available because of interfering compounds in the extract that co-elute with tripdiolide. Therefore, this paper describes a method wherein tripdiolide content can be measured from a small amount of the extract. The extract solution (600 µL) was applied into an aminopropyl SPE tube. Triptolide was eluted with dichloromethane:methanol (1 mL, 49:1 v/v), followed by tripdiolide elution with dichloromethane:methanol (3 mL, 17:3 v/v). The tripdiolide eluate was analysed by HPLC using an isocratic solvent system and was quantified by measuring the peak area at 219 nm. The contents of triptolide and tripdiolide in the extract were determined to be 807.32 ± 51.94 and 366.13 ± 17.21 µg/g of extract, respectively. Since tripdiolide is biologically active and makes up a considerable portion of the extract, for extract quality control and standardisation purposes, it should be measured along with triptolide using the proposed SPE-HPLC method.
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影响因子：
3.3
作者：
Song, XR;Yang, GL;Zhang, HY
通讯作者：
Zhang, HY
DOI：
10.1080/10826079408013632
发表时间：
1994-01-01
期刊：
JOURNAL OF LIQUID CHROMATOGRAPHY
影响因子：
--
作者：
CAI, JJ;TAO, XL;LIPSKY, PE
通讯作者：
LIPSKY, PE
影响因子：
3.8
作者：
Ma, Jun;Dey, Moul;Raskin, Ilya
通讯作者：
Raskin, Ilya
影响因子：
--
作者：
Tao, XL;Younger, J;Lipsky, PE
通讯作者：
Lipsky, PE
影响因子：
5
作者：
Lipsky, PE;Tao, XL
通讯作者：
Tao, XL