Amperometric immunosensor for rapid detection of Mycobacterium tuberculosis.
Amperometric immunosensor for rapid detection of Mycobacterium tuberculosis.
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DOI：
10.1088/0960-1317/25/5/055013
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Chung JH
中科院分区：
文献类型：
--
作者：
Hiraiwa M;Kim JH;Lee HB;Inoue S;Becker AL;Weigel KM;Cangelosi GA;Lee KH;Chung JH
结核病（TB）一直是一个主要的公共卫生问题，可以通过在低资源环境中使用准确和快速的诊断来更好地控制。一个简单的，便携式的，和灵敏的检测方法是需要点的护理（POC）设置。本文研究了一种电流型生物传感器-微探针免疫分析法，用于快速、低成本检测痰液中结核分枝杆菌。痰中的MTB被特异性地捕获在功能化的微尖端表面上并通过电流检测。数值研究表明，微尖表面电流信号随浸入深度的增加呈线性变化。使用参考微尖，浸没深度被补偿用于感测微尖。在微尖端表面上，目标细菌通过咖啡环效应被集中和组织，这放大了电流。为了提高信噪比，样品处理和冲洗步骤都使用去离子水作为安培测量的介质。当应用于加标到人痰液中的培养的MTB细胞时，检测限为100 CFU/mL，与先前报道的劳动密集型荧光检测方法相当。
Tuberculosis (TB) has been a major public health problem, which can be better controlled by using accurate and rapid diagnosis in low-resource settings. A simple, portable, and sensitive detection method is required for point-of-care (POC) settings. This paper studies an amperometric biosensor using a microtip immunoassay for a rapid and low cost detection of Mycobacterium Tuberculosis (MTB) in sputum. MTB in sputum is specifically captured on the functionalized microtip surface and detected by electric current. According to the numerical study, the current signal on microtip surface is linearly changed with increasing immersion depth. Using a reference microtip, the immersion depth is compensated for a sensing microtip. On the microtip surface, target bacteria are concentrated and organized by a coffee ring effect, which amplifies the electric current. To enhance the signal-to-noise ratio, both the sample processing- and rinsing steps are presented with use of deionized water as a medium for the amperometric measurement. When applied to cultured MTB cells spiked into human sputum, the detection limit was 100 CFU/mL, comparable to a more labor-intensive fluorescence detection method reported previously.
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影响因子：
3.7
作者：
Jassal MS;Nedeltchev GG;Lee JH;Choi SW;Atudorei V;Sharp ZD;Deretic V;Timmins GS;Bishai WR
通讯作者：
Bishai WR
影响因子：
7.4
作者：
Liu, Guodong;Lin, Ying-Ying;Lin, Yuehe
通讯作者：
Lin, Yuehe
影响因子：
--
作者：
Zhou L;He X;He D;Wang K;Qin D
通讯作者：
Qin D
影响因子：
4.2
作者：
Kim, Jong-Hoon;Shen, Amy Q.;Chung, Jae-Hyun
通讯作者：
Chung, Jae-Hyun
影响因子：
17.3
作者：
GRIFFITHS, D;HALL, G
通讯作者：
HALL, G