Scutellarein inhibits cancer cell metastasis in vitro and attenuates the development of fibrosarcoma in vivo.
Scutellarein inhibits cancer cell metastasis in vitro and attenuates the development of fibrosarcoma in vivo.
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黄芩素可在体外抑制癌细胞转移，并在体内减弱纤维肉瘤的发展。
DOI：
10.3892/ijmm.2014.1997
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发表时间：
2015-01
影响因子：
5.4
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Shi X;Chen G;Liu X;Qiu Y;Yang S;Zhang Y;Fang X;Zhang C;Liu X
纤维肉瘤是一种侵袭性和高度转移的结缔组织癌，有效的治疗方法有限。最近，人们对天然产物中的小分子化合物在癌症治疗中的作用重新产生了兴趣。在本研究中，我们研究了从多年生草本植物黄芩中提取的化合物黄芩苷（Scutellaria lateriflora），发现它具有抗癌潜力。细胞增殖实验和细胞周期分析显示，黄芩苷通过诱导细胞凋亡的方式显著抑制HT1080人纤维肉瘤细胞的增殖速率。此外，在Balb/c裸鼠的体内实验中，黄芩苷治疗后肿瘤的体积和重量明显减少。我们还利用HT1080细胞分析了黄芩苷对转移标志物的影响。结果表明，黄芩苷能有效抑制细胞迁移、侵袭及基质金属蛋白酶(MMP)-2、-9和-14的表达和活性。此外，由于黄芩苷介导的活化B细胞核因子κ-轻链增强子（NF-κB）活化下调，MMP活化和细胞存活受到抑制。综上所述，我们的数据表明，黄芩苷具有减缓纤维肉瘤发展和抑制癌细胞转移的能力。
Fibrosarcoma is an aggressive and highly metastatic cancer of the connective tissue, for which effective therapeutic methods are limited. Recently, there has been a renewed interest in small molecular compounds from natural products in the treatment of cancer. In the present study, we investigated the compound, scutellarein, extracted from the perennial herb Scutellaria lateriflora, and it was found to possess anticancer potential. Cell proliferation assay and cell cycle analysis revealed that the proliferation rate of HT1080 human fibrosarcoma cells was significantly suppressed by treatment with scutellarein through the induction of apoptosis. Moreover, an in vivo experiment using Balb/c nude mice revealed that the volume and weight of the tumors were markedly reduced following treatment with scutellarein. We also analyzed the effects of scutellarein on the markers of metastasis, using the HT1080 cells. The results indicated that scutellarein potently inhibited cell migration, invasion and the expression and activity of matrix metalloproteinase (MMP)-2, -9 and -14. Furthermore, MMP activation and cell survival were suppressed due to the scutellarein-mediated downregulation of nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) activation. In conclusion, our data suggest that scutellarein has the ability to attenuate the development of fibrosarcoma and inhibit cancer cell metastasis.
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