QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells.
QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells.
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DOI：
10.1093/jxb/err211
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发表时间：
2011-10
影响因子：
6.9
通讯作者：
Jiang L
中科院分区：
文献类型：
--
作者：
Miao Y;Li HY;Shen J;Wang J;Jiang L
果胶是复杂的多糖，是植物细胞壁的基本成分。在这项研究中，一种新的假定的拟南芥S-腺苷-L-甲硫氨酸（SAM）依赖性甲基转移酶，称为QUASIMODO 3（QUA 3，At 4g 00740），已被表征，并证明它是高尔基体本地化的，II型整合膜蛋白的果胶homogalacturonan（HG）的甲基化功能。虽然转基因拟南芥幼苗过表达，或敲低，QUA 3不显示改变表型或果胶甲基化的变化，这种酶是高度表达和丰富的拟南芥悬浮培养细胞。相比之下，在经历QUA 3 RNA干扰（RNAi）敲低的细胞中，果胶甲基化较少以及细胞壁多糖的组成和组装改变。两者合计，这些观察点高尔基体定位QUA 3在控制果胶甲基化和细胞壁生物合成在拟南芥悬浮细胞培养中起着至关重要的作用。
Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II integral membrane protein that functions in methylesterification of the pectin homogalacturonan (HG). Although transgenic Arabidopsis seedlings with overexpression, or knock-down, of QUA3 do not show altered phenotypes or changes in pectin methylation, this enzyme is highly expressed and abundant in Arabidopsis suspension-cultured cells. In contrast, in cells subjected to QUA3 RNA interference (RNAi) knock-down there is less pectin methylation as well as altered composition and assembly of cell wall polysaccharides. Taken together, these observations point to a Golgi-localized QUA3 playing an essential role in controlling pectin methylation and cell wall biosynthesis in Arabidopsis suspension cell cultures.
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影响因子：
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作者：
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通讯作者：
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