Purification of a new acidic glutathione S-transferase, GST-Yn1Yn1, with a high leukotriene-C4 synthase activity from rat brain.
Purification of a new acidic glutathione S-transferase, GST-Yn1Yn1, with a high leukotriene-C4 synthase activity from rat brain.
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从大鼠脑中纯化具有高白三烯-C4 合酶活性的新型酸性谷胱甘肽 S-转移酶 GST-Yn1Yn1。
DOI：
10.1111/j.1432-1033.1987.tb13681.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
K. Sato
中科院分区：
文献类型：
--
作者：
S. Tsuchida;T. Izumi;T. Shimizu;T. Ishikawa;I. Hatayama;K. Satoh;K. Sato
A new acidic form of glutathione S-transferase (GST, pI 6.2) was purified from rat brain by S-hexylglutathione affinity chromatography followed by chromatofocusing. This form occupied 20-25% of the total activity bound to the affinity column. It had a molecular mass (subunit 26 kDa) similar to that of a major GST form of rat testis (MT or 6-6) on sodium dodecyl sulfate/polyacrylamide gel electrophoresis. However, it differed from the MT in isoelectric point, activity towards 1,2-dichloro-4-nitrobenzene and immunological properties. On two-dimensional gel electrophoresis the brain form gave a spot which was identical in molecular mass, isoelectric point and immunological properties to a less acidic one (Yn1) of two spots (Yn1 and Yn2) of the testis GST-MT. Therefore, the brain acidic form is a homodimer, and named GST-Yn1Yn1. The activity was inhibited by sulfasalazine, an inhibitor of leukotriene-C4 synthase. This form (GST-Yn1Yn1) showed the highest leukotriene-C4 synthase activity, 496 nmol/mg protein in 5 min, among nine cytosolic GST isoenzymes from the rat. The Km values for leukotriene A4 and glutathione were 26 microM and 3.5 mM respectively. A major GST form of rat brain, occupying about 40% of the total activity, was identical with GST-P (7-7) purified from rat liver bearing preneoplastic hyperplastic nodules and localized at astroglias. GST-P also showed the significant leukotriene-C4 synthase activity, 67.2 nmol/mg protein in 5 min, but the Km for leukotriene A4 was 100 microM, fourfold higher than that of GST-Yn1 Yn1. These results suggest that mainly GST-Yn1 Yn1 may be involved in leukotriene-C4 synthesis in rat brain.
大鼠嗜碱性白血病细胞白三烯 C4 合成酶的分离和表征。
DOI：
10.1073/pnas.82.24.8399
发表时间：
1985
影响因子：
11.1
作者：
Yoshimoto,T;Soberman,RJ;Lewis,RA;Austen,KF
通讯作者：
Austen,KF
人脑的谷胱甘肽S-转移酶。
DOI：
10.1042/bj2250375
发表时间：
1985
期刊：
The Biochemical journal
影响因子：
--
作者：
Theodore,C;Singh,SV;Hong,TD;Awasthi,YC
通讯作者：
Awasthi,YC
由颗粒酶形成白三烯 C4 和 D4。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Jakschik,BA;Harper,T;Murphy,RC
通讯作者：
Murphy,RC