Lipid mediators are detectable in the nasal epithelium and differ by asthma status in female subjects.
Lipid mediators are detectable in the nasal epithelium and differ by asthma status in female subjects.
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DOI：
10.1016/j.jaci.2022.02.026
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发表时间：
2022-10
影响因子：
14.2
通讯作者：
Figueiredo, Camila A.
中科院分区：
文献类型：
--
作者：
Johnson, Randi K.;Manke, Jonathan;Campbell, Monica;Armstrong, Michael;Boorgula, Meher Preethi;Pinheiro, Gabriela;Santana, Cinthia Vila Nova;Mathias, Rasika A.;Barnes, Kathleen C.;Cruz, Alvaro;Reisdorph, Nichole;Figueiredo, Camila A.
脂质介体是多不饱和脂肪酸代谢的生物活性产物，在过敏性疾病中有助于炎症的启动和消退；然而，它们在与肺相关的生物样本中的存在尚未得到充分描述。我们的目标是量化鼻腔呼吸道上皮细胞中的脂质介质，并初步确定与哮喘的相关性。我们利用液-质联用技术，对11名哮喘门诊女性参与者和社区对照组(年龄30-55岁)的鼻黏膜上皮细胞样本中的56种脂质介质进行了初步研究。我们用描述性统计检验了每种化合物的存在，并用线性回归和偏最小二乘判别分析(PLS-DA)检验了脂质介质是否能区分哮喘(n=8)和对照组(n=3)。有关详细的方法，请参阅本文的在线资源库，网址为www.Jacion line.org。在所有样品中都检测到15种脂类介体，包括Resolvin D5(RVD5)，其中最高的中位浓度(以每μg蛋白的pg为单位)为13-HODE(126.481)、15-HETE(32.869)和13-OxoODE(13.251)。校正年龄的线性回归分析显示，重度哮喘患者的前列腺素E_2(PGE_2)浓度较对照组有升高趋势(P<；0.1)(平均差值为0.95，95%CI：−为0.04~1.95)。哮喘组的主成分3得分高于对照组(平均差值=2.42，95%CI：0.89~3.96)，表明15-HEPE、19，20-DiHDPA、RVD5、14-HDHA、17-HDHA和13-HOTrE的前拆分水平较低。在偏最小二乘法中，这些化合物中的大多数在区分哮喘病例和对照方面影响最大。鼻腔上皮中可以检测到脂质介质，它们的水平将哮喘患者与对照组区分开来。NA我们描述了鼻腔上皮细胞中的脂质介质浓度，并确定了区分哮喘患者和对照组的促分解分子的组合。测量这种组织中的这些化合物对于更大规模的哮喘研究是可行的。
Lipid mediators, bioactive products of polyunsaturated fatty acid metabolism, contribute to inflammation initiation and resolution in allergic diseases; however, their presence in lung-related biosamples has not been fully described. We aimed to quantify lipid mediators in the nasal airway epithelium and characterize preliminary associations with asthma. Using liquid chromatography mass-spectrometry, we conducted a pilot study to quantify 56 lipid mediators from nasal epithelial samples collected from 11 female participants of an outpatient asthma clinic and community controls (aged 30–55 years). We examined the presence of each compound using descriptive statistics to test whether lipid mediators could distinguish asthma (n=8) from control subjects (n=3) using linear regression and partial least squares-discriminant analysis (PLS-DA). For detailed Methods, please see this article’s Online Repository at www.jacionline.org. Fifteen lipid mediators were detectable in all samples, including Resolvin D5 (RVD5), with the highest median concentrations (in pg per μg protein) of 13-HODE (126.481), 15-HETE (32.869), and 13-OxoODE (13.251). From linear regression adjusted for age, prostaglandin E2 (PGE2) had a trend (p<0.1) for higher concentrations in severe asthma cases compared to controls (mean difference = 0.95, 95%CI: −0.04–1.95). Asthma cases had higher scores on principal component 3 compared to controls (mean difference = 2.42, 95%CI: 0.89–3.96), which represented lower levels of pro-resolving 15-HEPE, 19, 20-DiHDPA, RVD5, 14-HDHA, 17-HDHA, and 13-HOTrE. Most of these compounds were most influential in discriminating asthma cases from controls in PLS-DA. Lipid mediators are detectable in the nasal epithelium and their levels distinguish asthma cases from controls. NA We described lipid mediator concentrations in the nasal epithelium and identified combinations of pro-resolving molecules that discriminate asthma cases from controls. Measurement of these compounds in this tissue is feasible for larger asthma studies.
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影响因子：
4.1
作者：
Polinski KJ;Armstrong M;Manke J;Seifert J;Crume T;Yang F;Clare-Salzler M;Holers VM;Reisdorph N;Norris JM
通讯作者：
Norris JM
DOI：
10.1016/j.bbalip.2010.08.002
发表时间：
2010-12
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Bannenberg G;Serhan CN
通讯作者：
Serhan CN
影响因子：
6.1
作者：
Aggarwal, S.;Moodley, Y. P.;Misso, N. L.
通讯作者：
Misso, N. L.
影响因子：
2.4
作者：
Barker, M;Rayens, W
通讯作者：
Rayens, W
影响因子：
14.2
作者：
Imkamp, Kai;Berg, Marijn;Faiz, Alen
通讯作者：
Faiz, Alen