Comparison of a Shiga Toxin Enzyme-Linked Immunosorbent Assay and Two Types of PCR for Detection of Shiga Toxin-Producing Escherichia coli in Human Stool Specimens
Comparison of a Shiga Toxin Enzyme-Linked Immunosorbent Assay and Two Types of PCR for Detection of Shiga Toxin-Producing Escherichia coli in Human Stool Specimens
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志贺毒素酶联免疫吸附测定与两种 PCR 检测人粪便样本中产志贺毒素大肠杆菌的比较
DOI：
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发表时间：
2003
影响因子：
9.4
通讯作者：
F. Gunzer
中科院分区：
文献类型：
--
作者：
M. Pulz;A. Matussek;M. Monazahian;A. Tittel;E. Nikolic;M. Hartmann;T. Bellin;J. Buer;F. Gunzer
产志贺毒素（Stx）的大肠杆菌（STEC）是散发病例和世界范围内严重暴发的主要原因。疾病谱系包括轻度非出血性腹泻、出血性结肠炎和溶血性尿毒症综合征。STEC产生一个或多个stx，又分为Stx1和Stx2两大类。摄入受污染的食物或水、人际传播和与动物接触是主要的传播方式。产志贺毒素大肠杆菌的感染剂量可能少于100个微生物。感染的有效预防依赖于病原细菌的快速检测。在本研究中，我们通过三种不同的方法检测了295份粪便标本中产生Stx的大肠杆菌的存在：Stx酶联免疫吸附试验、传统PCR试验和LightCycler PCR （LC-PCR）试验方案，该方案最近由我们在汉诺威医学院医学微生物研究所的实验室开发。我们的目的是比较这三种方法，并检查产志贺毒素大肠杆菌LC-PCR方案在临床实验室的效用。在每个样本中加入一段对照DNA，以清楚地区分抑制样本和阴性样本，从而提高了LC-PCR方案的准确性。本研究结果表明，LC-PCR是一种快速、安全检测产志贺毒素大肠杆菌临床样品的有效工具。
ABSTRACT Shiga toxin (Stx)-producing Escherichia coli (STEC) is a major cause of sporadic cases of disease as well as serious outbreaks worldwide. The spectrum of illnesses includes mild nonbloody diarrhea, hemorrhagic colitis, and hemolytic-uremic syndrome. STEC produces one or more Stxs, which are subdivided into two major classes, Stx1 and Stx2. The ingestion of contaminated food or water, person-to-person spread, and contact with animals are the major transmission modes. The infective dose of STEC may be less than 100 organisms. Effective prevention of infection is dependent on rapid detection of the causative bacterial pathogen. In the present study, we examined 295 stool specimens for the presence of Stx-producing E. coli by three different methods: an Stx enzyme-linked immunosorbent assay, a conventional PCR assay, and a LightCycler PCR (LC-PCR) assay protocol recently developed by our laboratory at the Institute of Medical Microbiology at Hannover Medical School. Our intent was to compare these three methods and to examine the utility of the STEC LC-PCR protocol in a clinical laboratory. The addition of a control DNA to each sample to clearly discriminate inhibited specimens from negative ones enhanced the accuracy of the LC-PCR protocol. From our results, it can be concluded that LC-PCR is a very useful tool for the rapid and safe detection of STEC in clinical samples.
影响因子：
3.8
作者：
JACKSON, MP;NEWLAND, JW;OBRIEN, AD
通讯作者：
OBRIEN, AD
影响因子：
2.7
作者：
Wittwer, CT;Ririe, KM;Balis, UJ
通讯作者：
Balis, UJ