Fluorescent probes for live-cell RNA detection.
Fluorescent probes for live-cell RNA detection.
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DOI：
10.1146/annurev-bioeng-061008-124920
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发表时间：
2009
影响因子：
9.7
通讯作者：
Tsourkas A
中科院分区：
文献类型：
--
作者：
Bao G;Rhee WJ;Tsourkas A
分析基因表达的常用技术，例如聚合酶链式反应 (PCR)、微阵列和原位杂交，已被证明对于理解 RNA 加工和调控具有无价的价值。然而，这些技术依赖于裂解和/或固定细胞的使用，因此提供重要时空信息的能力受到限制。这导致了许多活细胞中 RNA 成像技术的发展，其中一些技术已经为了解 RNA 在决定细胞行为中的动态作用提供了重要的见解。在这里，我们回顾了可在活细胞中进行 RNA 成像的荧光探针，并讨论它们的实用性和局限性。还讨论了荧光探针面临的常见挑战，例如探针设计、递送和目标可及性。预计 RNA 活细胞成像的持续进步将为广泛的生物和医学应用带来新的、令人兴奋的机会。
Commonly used techniques for analyzing gene expression, such as polymerase chain reaction (PCR), microarrays, and in situ hybridization, have proven invaluable in understanding RNA processing and regulation. However, these techniques rely on the use of lysed and/or fixed cells and are therefore limited in their ability to provide important spatial-temporal information. This has led to the development of numerous techniques for imaging RNA in living cells, some of which have already provided important insight into the dynamic role RNA plays in dictating cell behavior. Here we review the fluorescent probes that have allowed for RNA imaging in living cells and discuss their utility and limitations. Common challenges faced by fluorescent probes, such as probe design, delivery, and target accessibility, are also discussed. It is expected that continued advancements in live cell imaging of RNA will open new and exciting opportunities in a wide range of biological and medical applications.
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影响因子：
14.9
作者：
Chen AK;Behlke MA;Tsourkas A
通讯作者：
Tsourkas A
影响因子：
3.9
作者：
BARRY, MA;EASTMAN, A
通讯作者：
EASTMAN, A
影响因子：
9.2
作者：
Glotzer, JB;Saffrich, R;Ephrussi, A
通讯作者：
Ephrussi, A
DOI：
10.1073/pnas.2233244100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Bratu, DP;Cha, BJ;Tyagi, S
通讯作者：
Tyagi, S
影响因子：
46.9
作者：
Dubertret, B;Calame, M;Libchaber, AJ
通讯作者：
Libchaber, AJ