Temporal and spatial regulation of ezrin-radixin-moesin-binding phosphoprotein-50-kDa (EBP50) during embryo implantation in mouse uterus.
Temporal and spatial regulation of ezrin-radixin-moesin-binding phosphoprotein-50-kDa (EBP50) during embryo implantation in mouse uterus.
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DOI：
10.3390/ijms131216418
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发表时间：
2012-12-03
影响因子：
5.6
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Li X;Xu WM;Yin TL;Zhao QH;Peng LY;Yang J
胚胎着床是妊娠成功的关键过程。到目前为止，胚胎着床的机制尚不清楚。EBP50是一种支架蛋白，已被证明在肿瘤的发生发展中起重要作用。胚胎植入和癌症的发展过程相似。因此，在本文中，我们利用免疫组织化学染色和免疫印迹分析来检测EBP50在小鼠胚胎着床过程中以及在其他相关模型中的时空表达和调控。我们发现EBP50在我们研究中使用的所有组的上皮细胞中都被检测到。在种植围术期，EBP50主要定位于顶膜。在妊娠第5天(D5)着床部位，EBP50主要表达在基质细胞的胞核中，而在第6天至第8天(D6~D8)，EBP50主要表达在蜕膜细胞的胞浆中。EBP50在假孕子宫中的表达无明显差异，在延迟着床激活的子宫中表达降低。人工蜕膜化也降低了EBP50的表达。因此，其表达水平和位置受着床窗口期活跃的胚泡和蜕膜的影响。
Embryo implantation is a crucial process for successful pregnancy. To date, the mechanism of embryo implantation remains unclear. Ezrin-radixin-moesin-binding protein-50-kDa (EBP50) is a scaffold protein, which has been shown to play an important role in cancer development. Embryo implantation and cancer follow a similar progression. Thus, in this article, we utilized immunohistochemical staining and western blot analyses to examine the spatiotemporal expression and regulation of EBP50 both in the mouse uterus during embryo implantation as well as in other related models. We found that EBP50 was detected in epithelial cells in all of the groups used in our study. During the peri-implantation period, EBP50 mainly localized in apical membranes. At the implantation site (IS) on day 5 (D5) of pregnancy, EBP50 was mainly expressed in the nuclei of stroma cells, whereas from day 6 to day 8 (D6–D8) of pregnancy, the expression of EBP50 was noted in the cytoplasm of decidual cells. The expression of EBP50 was not significantly different in the pseudopregnant uterus and decreased in the uteri subjected to activation of delayed implantation. Artificial decidualization also decreased EBP50 expression. Thus, the expression levels and location were affected by active blastocysts and decidualization during the window of implantation.
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