Immunohistochemical study of the early human fetal brain
Immunohistochemical study of the early human fetal brain
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早期人类胎儿大脑的免疫组织化学研究
DOI：
10.1007/bf00688096
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发表时间：
2004
影响因子：
12.7
通讯作者：
Y. Ishida
中科院分区：
文献类型：
--
作者：
A. Sasaki;J. Hirato;Y. Nakazato;Y. Ishida
为了探讨中枢神经系统的细胞遗传学，我们用抗波形蛋白、抗神经丝蛋白、抗神经元特异性烯醇化酶、抗胶质纤维酸性蛋白、抗S-100蛋白、抗亮氨酸7和抗髓鞘碱性蛋白抗体，对11例7~25周人胚胎和胎儿的脊髓和大脑进行了免疫组织化学研究。7周龄以上的大鼠心肌细胞内可见波形蛋白的表达。7周龄神经母细胞中可见NFP-68-kDa和-160-kDa成分。NFP(68 kDa和160 kDa)在8~9周主要分布在脊髓和大脑的边缘地带。7周时神经管内未发现NSE，而9周时脊髓和大脑均可见NSE。GFAP阳性细胞在第9周开始出现在脊髓，在第15周开始出现在大脑。S-100与胶质纤维酸性蛋白免疫反应基本一致。然而，S-100在更多的胶质母细胞中被观察到。Leu7在7周时被检测到，定位于中枢神经组织的神经束中。在这项研究中，MBP并不明显。我们的研究表明，在人脑中，神经元分化发生得比胶质细胞分化早得多，并且神经元和神经胶质细胞类别在早期人胎脑的脑室区并不共存。
SummaryTo assess the cytogenesis of the central nervous system we studied the spinal cord and the cerebrum in 11 human embryos and fetuses of gestation age 7–25 weeks immunohistochemically using anti-vimentin, anti-neurofilament protein (NFP), anti-neuron-specific enolase (NSE), anti-glial fibrillary acidic protein (GFAP), anti-S-100 protein, anti-Leu 7 and anti-myelin basic protein (MBP) antibodies. Vimentin was demonstrated in ventricular cells at 7 weeks and older. NFP-68-kDa and-160-kDa components were observed in neuroblastic cells of the neural tube at 7 weeks. NFP (68 and 160 kDa) was mainly located in the marginal zone of the spinal cord and the cerebrum at 8–9 weeks. NSE was not found in the neural tube at 7 weeks, although NSE was demonstrable at 9 weeks both in the spinal cord and in the cerebrum. GFAP-positive cells started to appear at 9 weeks in the spinal cord and at 15 weeks in the cerebrum, respectively. S-100 immunoreactivity was almost coincident with GFAP. S-100, however, was observed in more numerous glioblastic cells. Leu 7 was detected at 7 weeks and located in the neuropil of the central nervous tissue. MBP was not demonstrable in this study. Our study indicates that neuronal differentiation occurs much earlier than glial differentiation in the human brain and that neuronal and glial cell classes do not coexist in the ventricular zone of the early human fetal brain.
发育中的小鸡脊髓中的中间丝蛋白。
影响因子：
2.7
作者：
Tapscott,SJ;Bennett,GS;Toyama,Y;Kleinbart,F;Holtzer,H
通讯作者：
Holtzer,H