Goreisan Inhibits Upregulation of Aquaporin 4 and Formation of Cerebral Edema in the Rat Model of Juvenile Hypoxic-Ischemic Encephalopathy.
Goreisan Inhibits Upregulation of Aquaporin 4 and Formation of Cerebral Edema in the Rat Model of Juvenile Hypoxic-Ischemic Encephalopathy.
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DOI：
10.1155/2017/3209219
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Fukuda M
中科院分区：
文献类型：
--
作者：
Yano Y;Yano H;Takahashi H;Yoshimoto K;Tsuda S;Fujiyama K;Izumo-Shimizu Y;Motoie R;Ito M;Tanaka J;Ishii E;Fukuda M
继发性脑水肿调节在缺氧缺血性脑病（HIE）中具有预后意义，水通道蛋白4（AQP4）在脑水肿的发病机制中发挥重要作用。日本传统草药Goreisan可缓解成人脑水肿；然而，其对儿童的作用和药理机制尚不清楚。我们在幼年大鼠模型中研究了 Goreisan 对 HIE 相关脑水肿和 AQP4 表达的影响，该模型是通过大脑中动脉和颈总动脉联合闭塞建立的。磁共振成像显示，术后 24 小时和 48 小时，Goreisan（2 g/kg）治疗组的病变面积明显小于未治疗（盐水）组。术后36小时，与未治疗组相比，Goreisan组病变侧和非病变侧的AQP4 mRNA水平显着受到抑制。 Western blotting结果显示，与未治疗组相比，Goreisan组术后72小时病灶侧AQP4蛋白水平显着降低，但术后12小时或36小时无显着降低。 14 天后，Goreisan 组的存活率显着提高。这些发现表明，Goreisan 可能通过调节 AQP4 表达和功能来抑制 HIE 中的脑水肿并提高幼年大鼠的存活率。
Secondary cerebral edema regulation is of prognostic significance in hypoxic-ischemic encephalopathy (HIE), and aquaporin 4 (AQP4) plays an important role in the pathogenesis of cerebral edema. The traditional Japanese herbal medicine Goreisan relieves brain edema in adults; however, its effect and pharmacological mechanism in children are unknown. We investigated the effects of Goreisan on HIE-associated brain edema and AQP4 expression in a juvenile rat model, established by combined occlusion of middle cerebral and common carotid arteries. Magnetic resonance imaging showed that the lesion areas were significantly smaller in the Goreisan- (2 g/kg) treated group than in the nontreated (saline) group at 24 and 48 h postoperatively. AQP4 mRNA levels in the lesion and nonlesion sides were significantly suppressed in the Goreisan group compared with the nontreated group 36 h postoperatively. Western blotting revealed that levels of AQP4 protein were significantly decreased in the Goreisan group compared with the nontreated group in the lesion side 72 h postoperatively, but not at 12 or 36 h. After 14 days, the Goreisan group had a significantly better survival rate. These findings suggest that Goreisan suppresses brain edema in HIE and improves survival in juvenile rats, possibly via regulation of AQP4 expression and function.
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影响因子：
9.3
作者：
Tourdias T;Mori N;Dragonu I;Cassagno N;Boiziau C;Aussudre J;Brochet B;Moonen C;Petry KG;Dousset V
通讯作者：
Dousset V
Aquaporin-4 与脑血管疾病。
影响因子：
5.6
作者：
Chu H;Huang C;Ding H;Dong J;Gao Z;Yang X;Tang Y;Dong Q
通讯作者：
Dong Q
影响因子：
4.2
作者：
Ribeiro, Marlise de Castro;Hirt, Lorenz;Badaut, Jerome
通讯作者：
Badaut, Jerome
DOI：
10.1056/nejmoa1112066
发表时间：
2012-05-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Shankaran S;Pappas A;McDonald SA;Vohr BR;Hintz SR;Yolton K;Gustafson KE;Leach TM;Green C;Bara R;Petrie Huitema CM;Ehrenkranz RA;Tyson JE;Das A;Hammond J;Peralta-Carcelen M;Evans PW;Heyne RJ;Wilson-Costello DE;Vaucher YE;Bauer CR;Dusick AM;Adams-Chapman I;Goldstein RF;Guillet R;Papile LA;Higgins RD;Eunice Kennedy Shriver NICHD Neonatal Research Network
通讯作者：
Eunice Kennedy Shriver NICHD Neonatal Research Network
影响因子：
158.5
作者：
Azzopardi, Denis;Strohm, Brenda;Edwards, A. David
通讯作者：
Edwards, A. David