Determining the mechanical properties of plectin in mouse myoblasts and keratinocytes.
Determining the mechanical properties of plectin in mouse myoblasts and keratinocytes.
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DOI：
10.1016/j.yexcr.2014.10.001
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发表时间：
2015-02-15
影响因子：
3.7
通讯作者：
Goldmann, Wolfgang H.
中科院分区：
文献类型：
--
作者：
Bonakdar, Navid;Schilling, Achim;Spoerrer, Marina;Lennert, Pablo;Mainka, Astrid;Winter, Lilli;Walko, Gernot;Wiche, Gerhard;Fabry, Ben;Goldmann, Wolfgang H.
关键词：
凝集素是基于中间丝（IF）的细胞连接蛋白的原型。它通过相互连接和锚定细胞骨架丝来机械地影响细胞，并作为信号蛋白的支架和对接平台来控制细胞骨架动力学。由人类plectin基因突变引起的最常见的疾病，单纯性大疱性表皮病伴肌营养不良症（EBS-MD），其特征在于严重的皮肤起泡和进行性肌营养不良。因此，我们比较了生物力学特性和对机械应力的反应，小鼠plectin缺陷型成肌细胞和角质形成细胞与野生型细胞。使用细胞拉伸装置，与野生型细胞相比，缺乏plectin的成肌细胞在外部应力下表现出较低的机械脆弱性，这归因于较低的细胞预应力。相反，成肌细胞，野生型和plectin缺乏角质形成细胞没有显着差异。在磁性镊子测量使用纤连蛋白涂层的顺磁珠，角质形成细胞的刚度高于成肌细胞。有趣的是，细胞硬度，粘附强度和细胞骨架动力学显着改变，在plectin缺乏相比，野生型成肌细胞，而较小的差异之间观察到plectin缺乏和野生型角质形成细胞，表明plectin可能是更重要的稳定细胞骨架结构的成肌细胞比角质形成细胞。牵引力与缺乏果胶和野生型成肌细胞和角质形成细胞的硬度密切相关。与此相反，细胞运动性是可比的，在plectin缺乏和野生型成肌细胞，但显着增加plectin缺乏相比，野生型角质形成细胞。因此，我们假设，缺乏网蛋白的生物力学性能取决于各自的细胞类型有不同的影响。中间体粘附相关蛋白质类素具有不同的生物力学影响，这取决于细胞/组织类型。在plectin−/−成肌细胞中，细胞的脆弱性、刚度、应变和结合强度低于野生型细胞。Plectin−/−角质形成细胞比野生型细胞表现出更高的细胞刚度、结合强度、应变和速度。
Plectin is the prototype of an intermediate filament (IF)-based cytolinker protein. It affects cells mechanically by interlinking and anchoring cytoskeletal filaments and acts as scaffolding and docking platform for signaling proteins to control cytoskeleton dynamics. The most common disease caused by mutations in the human plectin gene, epidermolysis bullosa simplex with muscular dystrophy (EBS-MD), is characterized by severe skin blistering and progressive muscular dystrophy. Therefore, we compared the biomechanical properties and the response to mechanical stress of murine plectin-deficient myoblasts and keratinocytes with wild-type cells. Using a cell stretching device, plectin-deficient myoblasts exhibited lower mechanical vulnerability upon external stress compared to wild-type cells, which we attributed to lower cellular pre-stress. Contrary to myoblasts, wild-type and plectin-deficient keratinocytes showed no significant differences. In magnetic tweezer measurements using fibronectin-coated paramagnetic beads, the stiffness of keratinocytes was higher than of myoblasts. Interestingly, cell stiffness, adhesion strength, and cytoskeletal dynamics were strikingly altered in plectin-deficient compared to wild-type myoblasts, whereas smaller differences were observed between plectin-deficient and wild-type keratinocytes, indicating that plectin might be more important for stabilizing cytoskeletal structures in myoblasts than in keratinocytes. Traction forces strongly correlated with the stiffness of plectin-deficient and wild-type myoblasts and keratinocytes. Contrary to that cell motility was comparable in plectin-deficient and wild-type myoblasts, but was significantly increased in plectin-deficient compared to wild-type keratinocytes. Thus, we postulate that the lack of plectin has divergent implications on biomechanical properties depending on the respective cell type. The intermediate filament-associated protein plectin has divergent biomechanical implications depending on cell/tissue type. In plectin−/− myoblasts, cell vulnerability, stiffness, strain and binding strength are lower than in wild-type cells. Plectin−/− keratinocytes exhibit higher cell stiffness, binding strength, strain and velocity than wild-type cells.
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DOI：
10.1083/jcb.200711058
发表时间：
2008-05-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Konieczny P;Fuchs P;Reipert S;Kunz WS;Zeöld A;Fischer I;Paulin D;Schröder R;Wiche G
通讯作者：
Wiche G
影响因子：
6.5
作者：
Seltmann, Kristin;Roth, Wera;Kroeger, Cornelia;Loschke, Fanny;Lederer, Marcell;Huettelmaier, Stefan;Magin, Thomas M.
通讯作者：
Magin, Thomas M.
影响因子：
1.6
作者：
Pelham, RJ;Wang, YL
通讯作者：
Wang, YL
影响因子：
3.4
作者：
Kollmannsberger, Philip;Mierke, Claudia Tanja;Fabry, Ben
通讯作者：
Fabry, Ben
影响因子：
1.6
作者：
Kollmannsberger, Philip;Fabry, Ben
通讯作者：
Fabry, Ben