Repeated pre-training sleep restriction in adolescent rats impaired spatial performance
Repeated pre-training sleep restriction in adolescent rats impaired spatial performance
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青春期大鼠反复训练前睡眠限制损害空间表现
DOI：
10.1007/s41105-016-0080-8
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发表时间：
2017
影响因子：
1.1
通讯作者：
Ye Chen-Bo
中科院分区：
文献类型：
--
作者：
Yang Su-Rong;Sun Huan-Xin;Hu Zhen-Zhen;Wang Si-Heng;Sun Hui;Xue Yin-Jia;Ye Chen-Bo
慢性睡眠剥夺（SD）是青少年学生普遍存在的问题。首先，我们研究了不同水平的训练前SD对青春期大鼠空间操作能力的影响。大鼠连续15天接受2或4小时/天的SD。在实验第9-15天SD后立即进行Morris水迷宫测试。在第一个训练日的最后3次试验中，与其各自的对照动物相比，先前SD 4 h但非SD 2 h的大鼠显示出在没有引导的情况下着陆平台的试验百分比（着陆率）显著降低。在6天的训练过程中，4 h SD组大鼠比对照组大鼠定位平台的时间更长，而2 h SD组大鼠定位平台的时间更短。在探索实验中，SD 4 h和SD 2 h的大鼠在目标象限内停留的时间和平台穿越次数与其各自的对照组无显著差异。这些结果表明，慢性前4小时SD，而不是2小时SD，损害青春期大鼠的空间获取能力。其次，我们探讨了石杉碱甲（Hup A），这已被证明是改善痴呆的认知障碍，是否可以防止慢性4小时SD诱导的青春期大鼠的学习能力下降。在4 h SD结束前30 min，通过管饲法给予Hup A（0.1 mg/kg/天）。hup A治疗后，降低的落地率增加，延长的逃避潜伏期和距离缩短，提示hup A对预防青少年反复睡眠限制所致的空间认知障碍有一定的作用。
Chronic sleep deprivation (SD) is an overwhelming problem in young students. Firstly, we investigated whether different levels of pre-training SD had effects on spatial performance in adolescent rats. Rats were subjected to 2 or 4 h/day SD for 15 consecutive days. Morris water maze tests were conducted immediately after SD on experimental days 9–15. During the last 3 trials on the first training day, compared with their respective control animals, the rats with prior 4 h SD, but not 2 h SD, showed a significant reduction in percentage of the trials landing the platform without guidance (landing rate). During the whole 6 training days, the rats subjected to 4 h SD, not 2 h SD, spent longer time to locate the platform than the control rats did. In the probe test, the rats with 4 h SD and 2 h SD showed no significant difference with their respective control in time spent in the target quadrant and numbers of platform crossings. These results indicated that chronic prior 4 h SD, but not 2 h SD, impaired spatial acquisition capability in adolescent rats. Secondly, we explored whether huperzine A (Hup A), which has been proved to improve cognitive impairment in dementia, could prevent adolescent rats from chronic 4 h SD-induced learning decline. Hup A (0.1 mg/kg/day) was administered by gavage 30 min before the end of 4 h SD. With hup A treatment, the reduced landing rate was increased and the prolonged escape latency and distance were shortened, which suggested that hup A was promising in preventing the spatial cognitive deficits induced by repeated sleep restriction in adolescents.
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DOI：
10.1080/09168451.2015.1052773
发表时间：
2015-06
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
--
作者：
Takuya Ohba;Y. Yoshino;M. Ishisaka;N. Abe;K. Tsuruma;M. Shimazawa;M. Oyama;T. Tabira;H. Hara
通讯作者：
Takuya Ohba;Y. Yoshino;M. Ishisaka;N. Abe;K. Tsuruma;M. Shimazawa;M. Oyama;T. Tabira;H. Hara
影响因子：
5.3
作者：
Soto-Rodriguez S;Lopez-Armas G;Luquin S;Ramos-Zuñiga R;Jauregui-Huerta F;Gonzalez-Perez O;Gonzalez-Castañeda RE
通讯作者：
Gonzalez-Castañeda RE
影响因子：
15.9
作者：
Kirszenblat L;van Swinderen B
通讯作者：
van Swinderen B
影响因子：
16.2
作者：
Tononi G;Cirelli C
通讯作者：
Cirelli C
影响因子：
4.4
作者：
Hagewoud, Roelina;Havekes, Robbert;Meerlo, Peter
通讯作者：
Meerlo, Peter