Equal distribution of lipid droplets in daughter cells is regulated by microtubules.
Equal distribution of lipid droplets in daughter cells is regulated by microtubules.
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DOI：
10.1080/15384101.2023.2210335
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发表时间：
2023-06
期刊：
影响因子：
4.3
通讯作者：
Ren, Zhuqing
中科院分区：
文献类型：
--
作者：
Huang, Jin;Jin, Yi;Yu, Ziwei;Zhang, Junzhi;Jian, Wu;Ren, Zhuqing
在真核细胞分裂过程中，细胞器通过动态过程分布在子细胞之间，以确保细胞能够分化并正确执行其功能。揭示脂滴（LD）的分布模式可能有助于揭示细胞分裂过程中膜重塑和脂滴功能的机制。我们的结果表明，在胞质分裂过程中，LD 在两个子细胞中均匀分布。进一步的实验表明，调节LD运动的关键因素是微管（MT）驻留蛋白KIF5B。由于KIF5B结构缺乏亲水区域，我们相信存在介导LD和KIF5B之间相互作用的蛋白质。对 LD 表面 KIF5B 相互作用蛋白的质谱检测表明，LD 首先被中间丝包裹形成网状结构，然后与 MT 接触以介导胞质分裂过程中的脂滴运动。 LD 均匀分布的破坏可能会阻碍细胞增殖，甚至导致细胞凋亡。
During eukaryotic cell division, organelles are distributed between daughter cells through a dynamic process to ensure that cells can differentiate and perform their functions correctly. Uncovering the mode of lipid droplet (LD) distribution may help reveal the mechanism of membrane remodeling during cell division and lipid droplet function. Our results showed that LDs were equally distributed in both daughter cells during cytokinesis. Further experiments demonstrated that the key factor regulating the movement of LDs is the microtubule (MT)-resident protein KIF5B. Because the KIF5B structure lacks a hydrophilic region, we believe that there are proteins that mediate the interaction between LDs and KIF5B. Mass spectrometric detection of KIF5B-interacting proteins on the surface of LDs demonstrated that LDs were first wrapped by intermediate filaments forming a meshwork and then contacted with MTs to mediate lipid droplet movement during cytokinesis. Disruption of the homogeneous distribution of LDs may hinder cell proliferation and even lead to apoptosis.
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