Lipid droplets in mammalian eggs are utilized during embryonic diapause.
Lipid droplets in mammalian eggs are utilized during embryonic diapause.
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DOI：
10.1073/pnas.2018362118
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发表时间：
2021-03-09
影响因子：
11.1
通讯作者：
Ptak GE
中科院分区：
文献类型：
--
作者：
Arena R;Bisogno S;Gąsior Ł;Rudnicka J;Bernhardt L;Haaf T;Zacchini F;Bochenek M;Fic K;Bik E;Barańska M;Bodzoń-Kułakowska A;Suder P;Depciuch J;Gurgul A;Polański Z;Ptak GE
在整个植入前发育过程中，脂滴（LD）储存在胚胎中。然而，LDs 在胚胎中的作用仍然未知。胚胎滞育（ED）是胚胎在等待子宫容受信号植入时的暂时停滞。我们提供的证据表明 LD 在维持 ED 方面发挥着至关重要的作用。滞育胚胎表现出外泌体释放增加，反映了向母亲发出的胚胎信号增强。在 ED 期间，脂质的减少是由碳水化合物代谢转变为脂质分解代谢引起的。我们还表明，各种哺乳动物卵母细胞中 LD 的存在与其物种特异性滞育长度呈正相关。我们的结果揭示了 LD 在胚胎发育中的功能作用。胚胎滞育（ED）是胚胎在囊胚阶段等待子宫容受信号植入时的暂时停滞。当子宫接受信号受阻或延迟时，100 多个物种所使用的 ED 也可能发生在正常“非滞育”哺乳动物中。在植入前胚胎发育的整个过程中储存了大量的脂滴（LD），但不同哺乳动物物种的脂质量差异很大。然而，LDs 在哺乳动物卵子和胚胎中的作用仍然未知。在这里，我们使用小鼠模型提供了证据，证明 LD 在维持 ED 方面发挥着至关重要的作用。通过从受精卵中机械去除 LD，我们证明脱脂胚胎在 ED 期间无法存活。在没有 ED 的情况下，LD 对于正常的即时着床来说并不是必需的。我们进一步证明，随着 ED 的进展，细胞内脂质的量减少，并且成分发生变化。这种脂质的减少是由滞育囊胚中从碳水化合物代谢转变为脂质分解代谢引起的，囊胚也表现出外泌体释放增加，反映了向母体发出的胚胎信号增强。我们还表明，各种哺乳动物卵母细胞中 LD 的存在与其物种特异性滞育长度呈正相关。我们的结果揭示了 LD 在胚胎发育中的功能作用。这些结果有助于开发诊断技术和治疗复发性着床失败，并可能引发发育生物学和生殖医学领域的进一步研究。
Lipid droplets (LDs) are stored in the embryo throughout the preimplantation development. Yet, the role of LDs in the embryo remains unknown. Embryonic diapause (ED) is a temporary arrest of an embryo when it waits for the uterine receptivity signal to implant. We provide evidence that LDs play a crucial role in maintaining ED. Diapausing embryos exhibit increased release of exosomes reflecting elevated embryonic signaling to the mother. During ED, the decrease in lipid is caused by a switch from carbohydrate metabolism to lipid catabolism. We have also shown that presence of LDs in the oocytes of various mammals positively corelates with their species-specific length of diapause. Our results reveal the functional role of LDs in embryonic development. Embryonic diapause (ED) is a temporary arrest of an embryo at the blastocyst stage when it waits for the uterine receptivity signal to implant. ED used by over 100 species may also occur in normally “nondiapausing” mammals when the uterine receptivity signal is blocked or delayed. A large number of lipid droplets (LDs) are stored throughout the preimplantation embryo development, but the amount of lipids varies greatly across different mammalian species. Yet, the role of LDs in the mammalian egg and embryo remains unknown. Here, using a mouse model, we provide evidence that LDs play a crucial role in maintaining ED. By mechanical removal of LDs from zygotes, we demonstrated that delipidated embryos are unable to survive during ED. LDs are not essential for normal prompt implantation, without ED. We further demonstrated that with the progression of ED, the amount of intracellular lipid reduces, and composition changes. This decrease in lipid is caused by a switch from carbohydrate metabolism to lipid catabolism in diapausing blastocysts, which also exhibit increased release of exosomes reflecting elevated embryonic signaling to the mother. We have also shown that presence of LDs in the oocytes of various mammals positively corelates with their species-specific length of diapause. Our results reveal the functional role of LDs in embryonic development. These results can help to develop diagnostic techniques and treatment of recurrent implantation failure and will likely ignite further studies in developmental biology and reproductive medicine fields.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
囊胚的整体蛋白质组学分析揭示了控制小鼠胚胎滞育和植入重新激活的关键分子机制
影响因子：
3.6
作者：
Fu, Zheng;Wang, Bingyan;Wang, Haibin
通讯作者：
Wang, Haibin
DOI：
10.1073/pnas.0402597101
发表时间：
2004-07-13
影响因子：
11.1
作者：
Hamatani, T;Daikoku, T;Dey, SK
通讯作者：
Dey, SK
DOI：
10.1007/bf01033631
发表时间：
1982-01-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
ANGERMULLER, S;FAHIMI, HD
通讯作者：
FAHIMI, HD
影响因子：
7.3
作者：
Cha J;Dey SK
通讯作者：
Dey SK