Molecular fingerprinting of nanoparticles in complex media with non-contact photoacoustics: beyond the light scattering limit.
Molecular fingerprinting of nanoparticles in complex media with non-contact photoacoustics: beyond the light scattering limit.
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DOI：
10.1038/s41598-018-32580-2
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发表时间：
2018-09-26
影响因子：
4.6
通讯作者：
O'Donnell M
中科院分区：
文献类型：
--
作者：
Pelivanov I;Petrova E;Yoon SJ;Qian Z;Guye K;O'Donnell M
光学仪器甚至可以探测到单个分子的水平。特别是，每个分子、溶液和结构（如活细胞）都具有代表分子指纹的独特吸收光谱。这种光谱可以帮助从其他分子中识别特定分子或量化其浓度;然而，散射限制了复杂化合物中的分子指纹，必须克服。在这里，我们提出了一种新的，非接触式光声（PA）为基础的方法，几乎可以完全消除背景光散射的影响，吸收测量异质性高散射的解决方案，此外，分离的内在吸收纳米级的物体从他们的散射。特别是，我们测量纯吸收光谱的解决方案的金纳米棒（GNRs）作为一个例子的等离子体激元剂，并表明这些光谱与传统的紫外-可见分光光度法测量的消光不同。最后，我们展示了当纳米颗粒被细胞内化时，原始GNR吸收如何变化。
Optical instruments can probe physical systems even to the level of individual molecules. In particular, every molecule, solution, and structure such as a living cell has a unique absorption spectrum representing a molecular fingerprint. This spectrum can help identify a particular molecule from others or quantify its concentration; however, scattering limits molecular fingerprinting within a complex compound and must be overcome. Here, we present a new, non-contact photoacoustic (PA)-based method that can almost completely remove the influence of background light scattering on absorption measurements in heterogeneous highly scattering solutions and, furthermore, separate the intrinsic absorption of nanoscale objects from their scattering. In particular, we measure pure absorption spectra for solutions of gold nanorods (GNRs) as an example of a plasmonic agent and show that these spectra differ from the extinction measured with conventional UV-VIS spectrophotometry. Finally, we show how the original GNR absorption changes when nanoparticles are internalized by cells.
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影响因子：
4.6
作者：
Kim SE;Lee BR;Lee H;Jo SD;Kim H;Won YY;Lee J
通讯作者：
Lee J
来自等离子体纳米颗粒的光声波的环境依赖性产生。
影响因子：
13.3
作者：
Chen, Yun-Sheng;Frey, Wolfgang;Aglyamov, Salavat;Emelianov, Stanislav
通讯作者：
Emelianov, Stanislav
影响因子：
3.3
作者：
Jain, PK;Lee, KS;El-Sayed, MA
通讯作者：
El-Sayed, MA
影响因子：
3.9
作者：
Joplin, Anneli;Chang, Wei-Shun;Link, Stephan
通讯作者：
Link, Stephan
影响因子：
1.9
作者：
Khokhlova, Tatiana D.;Pelivanov, Ivan M.;Karabutov, Alexander A.
通讯作者：
Karabutov, Alexander A.