SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells.
SARS-CoV replicates in primary human alveolar type II cell cultures but not in type I-like cells.
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DOI：
10.1016/j.virol.2007.09.045
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发表时间：
2008-03-01
期刊：
影响因子：
3.7
通讯作者：
Mason, Robert J.
中科院分区：
文献类型：
--
作者：
Mossel, Eric C.;Wang, Jieru;Jeffers, Scott;Edeen, Karen E.;Wang, Shuanglin;Cosgrove, Gregory P.;Funk, C. Joel;Manzer, Rizwan;Miura, Tanya A.;Pearson, Leonard D.;Holmes, Kathryn V.;Mason, Robert J.
严重急性呼吸系统综合征（SARS）是一种以弥漫性肺泡损伤为特征的疾病。我们分离了人肺泡II型细胞，并使其保持高度分化状态。通过RT-PCR检测病毒亚基因组RNA和病毒滴度的增加，ⅱ型细胞培养支持SARS-CoV复制。病毒滴度在24小时内达到最大值，峰值约为105 pfu/mL。培养物中的两种细胞类型受到感染。一种细胞类型为II型细胞，SP-A、SP-C、细胞角蛋白（一种II型细胞特异性单克隆抗体）和Ep-CAM阳性。另一种细胞类型由梭形细胞组成，呈波形蛋白和胶原III阳性，可能是成纤维细胞。在i型细胞和巨噬细胞中未检测到病毒复制。因此，分化的成人肺泡II型细胞具有传染性，但肺泡i型样细胞和肺泡巨噬细胞不支持生产性感染。
Severe acute respiratory syndrome (SARS) is a disease characterized by diffuse alveolar damage. We isolated human alveolar type II cells and maintained them in a highly differentiated state. Type II cell cultures supported SARS-CoV replication as evidenced by RT-PCR detection of viral subgenomic RNA and an increase in virus titer. Virus titers were maximal by 24 h and peaked at approximately 105 pfu/mL. Two cell types within the cultures were infected. One cell type was type II cells, which were positive for SP-A, SP-C, cytokeratin, a type II cell-specific monoclonal antibody, and Ep-CAM. The other cell type was composed of spindle-shaped cells that were positive for vimentin and collagen III and likely fibroblasts. Viral replication was not detected in type I-like cells or macrophages. Hence, differentiated adult human alveolar type II cells were infectible but alveolar type I-like cells and alveolar macrophages did not support productive infection.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
DOI：
10.1074/jbc.m508381200
发表时间：
2006-02-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Huang IC;Bosch BJ;Li F;Li W;Lee KH;Ghiran S;Vasilieva N;Dermody TS;Harrison SC;Dormitzer PR;Farzan M;Rottier PJ;Choe H
通讯作者：
Choe H
影响因子：
11.8
作者：
Kaye M
通讯作者：
Kaye M
影响因子：
5.4
作者：
Sims, AC;Baric, RS;Pickles, RJ
通讯作者：
Pickles, RJ
影响因子：
7.3
作者：
He, L.;Ding, Y.;Jiang, S.
通讯作者：
Jiang, S.