Single nuclei transcriptomics in human and non-human primate striatum in opioid use disorder.
Single nuclei transcriptomics in human and non-human primate striatum in opioid use disorder.
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DOI：
10.1038/s41467-024-45165-7
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发表时间：
2024-01-31
影响因子：
16.6
通讯作者：
Logan, Ryan W.
中科院分区：
文献类型：
--
作者：
Phan, BaDoi N.;Ray, Madelyn H.;Xue, Xiangning;Fu, Chen;Fenster, Robert J.;Kohut, Stephen J.;Bergman, Jack;Haber, Suzanne N.;Mccullough, Kenneth M.;Fish, Madeline K.;Glausier, Jill R.;Su, Qiao;Tipton, Allison E.;Lewis, David A.;Freyberg, Zachary;Tseng, George C.;Russek, Shelley J.;Alekseyev, Yuriy;Ressler, Kerry J.;Seney, Marianne L.;Pfenning, Andreas R.;Logan, Ryan W.
在大脑中，纹状体是参与奖赏和目标导向行为的异质性区域。纹状体功能障碍与精神疾病有关，包括阿片类药物使用障碍（OUD）。根据神经解剖学，纹状体亚区被划分，每个亚区在OUD中具有独特的作用。在OUD中，背侧纹状体参与改变奖励处理，习惯的形成和退缩期间负面情绪的发展。使用单核RNA测序，我们鉴定了两种典型的（例如，多巴胺受体亚型）和丰度较低的细胞群（例如，interneurons）中。与神经变性、干扰素反应和DNA损伤相关的通路在OUD个体的纹状体神经元中显著富集。在阿片类药物暴露的恒河猴的纹状体神经元中，DNA损伤标记物也升高。与慢性应激相关的胶质细胞亚型的性别特异性分子差异在OUD中发现，特别是女性个体。总之，我们描述了不同类型的细胞在人类背侧纹状体，并确定细胞类型特异性改变OUD。背侧纹状体（一个对奖赏和习惯很重要的大脑区域）的功能障碍与阿片类药物使用障碍（OUD）有关。在这里，作者描绘了人类背侧纹状体的不同细胞群，揭示了OUD中炎症和DNA损伤信号的改变。
In brain, the striatum is a heterogenous region involved in reward and goal-directed behaviors. Striatal dysfunction is linked to psychiatric disorders, including opioid use disorder (OUD). Striatal subregions are divided based on neuroanatomy, each with unique roles in OUD. In OUD, the dorsal striatum is involved in altered reward processing, formation of habits, and development of negative affect during withdrawal. Using single nuclei RNA-sequencing, we identified both canonical (e.g., dopamine receptor subtype) and less abundant cell populations (e.g., interneurons) in human dorsal striatum. Pathways related to neurodegeneration, interferon response, and DNA damage were significantly enriched in striatal neurons of individuals with OUD. DNA damage markers were also elevated in striatal neurons of opioid-exposed rhesus macaques. Sex-specific molecular differences in glial cell subtypes associated with chronic stress were found in OUD, particularly female individuals. Together, we describe different cell types in human dorsal striatum and identify cell type-specific alterations in OUD. Dysfunction in dorsal striatum, a brain region important for reward and habits, is linked to opioid use disorder (OUD). Here, authors delineate diverse cell populations in human dorsal striatum, revealing altered inflammatory and DNA damage signaling in OUD.
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影响因子：
4.2
作者：
Bachtell RK;Jones JD;Heinzerling KG;Beardsley PM;Comer SD
通讯作者：
Comer SD
影响因子：
5.3
作者：
Yang, Jenq-Lin;Sykora, Peter;Wilson, David M., III;Mattson, Mark P.;Bohr, Vilhelm A.
通讯作者：
Bohr, Vilhelm A.
影响因子：
4.7
作者：
Barko, Kelly;Shelton, Micah;Xue, Xiangning;Afriyie-Agyemang, Yvette;Puig, Stephanie;Freyberg, Zachary;Tseng, George C.;Logan, Ryan W.;Seney, Marianne L.
通讯作者：
Seney, Marianne L.
DOI：
10.1093/bioinformatics/btaa1009
发表时间：
2021-04-05
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Ahlmann-Eltze C;Huber W
通讯作者：
Huber W
影响因子：
6.9
作者：
Deak JD;Johnson EC
通讯作者：
Johnson EC