Elastase-induced lung emphysema in rats is not reduced by hematopoietic growth factors when applied preventionally
Elastase-induced lung emphysema in rats is not reduced by hematopoietic growth factors when applied preventionally
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预防性应用时，造血生长因子不会减少弹性蛋白酶诱导的大鼠肺气肿
DOI：
10.1007/s00428-008-0591-z
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发表时间：
2008
期刊：
影响因子：
3.5
通讯作者：
J. Hohlfeld
中科院分区：
文献类型：
--
作者：
A. Schmiedl;Tobias Lempa;H. Hoymann;S. Rittinghausen;D. Popa;T. Tschernig;H. Fehrenbach;R. Pabst;M. Hoeper;J. Hohlfeld
我们假设，先前激活干细胞后，肺气肿的形成可能会减少。动物在气管内注入弹性酶或赋形剂前3天开始每日给予造血生长因子(GF；重组大鼠干细胞因子+重组粒细胞集落刺激因子；n = 6，弹性酶/GF组)或赋形剂(n = 9，弹性酶/假手术组)，并持续25天。对照组动物给予生理盐水(n = 9，假/假手术组)。第25天，将2毫升的泵植入所有动物的皮下，输送200gμg/h的5-溴-2-脱氧尿苷(BrdU)，直到研究结束。与对照组相比，弹性酶/假手术组的总肺活量(TLC)和功能残气量(FRC)显著增加，平均游离肺泡通道数、肺泡体积显著增加，肺间隔密度显著降低。弹性蛋白酶/GF组TLC和FRC显著增加，肺泡大小和体积显著增加，隔膜密度、体积和厚度显著减小。弹性酶/假手术组和弹性酶/GF组肺实质和终末细支气管处的增殖仍显著减少。弹性蛋白酶/GF组的血细胞数量显著增加。应用GF增强的肺气肿可能是因为炎症活动增加，这是预防性治疗的结果。
We hypothesized that formation of pulmonary emphysema could be diminished after previous activation of stem cells. Animals received either a daily dose of the hematopoietic growth factors (GF; recombinant rat stem cell factor plus recombinant granulocyte colony stimulating factor; n = 6, Elastase/GF group) or vehicle (n = 9, Elastase/Sham group) starting 3 days before intratracheal instillation of elastase or vehicle and continued for another 25 days. Control animals were treated with NaCl (n = 9, Sham/Sham group). On day 25, in all animals, a 2-mL pump was implanted subcutaneously that delivered 200 μg/h 5-bromo-2-desoxyuridine (BrdU) until study termination. Compared to controls, the Elastase/Sham group exhibited elevated total lung capacity (TLC) and functional residual capacity (FRC), significantly increased mean free alveolar pathway, alveolar volume, and decreased septal density. The Elastase/GF group showed (1) a significant increase of TLC and FRC, (2) a significant increase in alveolar size and volume, (3) a significant reduction of septal density, volume, and thickness. Proliferation in lung parenchyma and in terminal bronchioles remained significantly decreased in the Elastase/Sham group and the Elastase/GF group. Blood cell number has significantly increased in the Elastase/GF group.The application of GF-enhanced pulmonary emphysema, presumable because of increased inflammatory activity, was a result of a preventive treatment.
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弹性蛋白酶诱导的肺气肿中肺结缔组织成分的平衡。
DOI：
--
发表时间：
1983
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Karlinsky,J;Fredette,J;Davidovits,G;Catanese,A;Snider,R;Faris,B;Snider,GL;Franzblau,C
通讯作者：
Franzblau,C
DOI：
10.1152/ajplung.2000.278.5.l955
发表时间：
2000-05-01
影响因子：
4.9
作者：
Massaro, GD;Massaro, D
通讯作者：
Massaro, D
影响因子：
15.9
作者：
Zheng, T;Zhu, Z;Elias, JA
通讯作者：
Elias, JA
DOI：
10.1165/rcmb.2005-0175rc
发表时间：
2005-10-01
影响因子：
6.4
作者：
Kotton, DN;Fabian, AJ;Mulligan, RC
通讯作者：
Mulligan, RC